Macamide B对缺氧缺血性脑损伤新生小鼠脑中雌激素受体表达的影响

目的:观察玛卡酰胺B(Macamide B)对缺氧缺血性脑损伤(HIBD)新生小鼠脑组织中雌激素受体α(ER α)和雌激素受体β(ER β)表达的影响。方法:选择出生7 d C57BL/6小鼠30只并将其分为3组:假手术组(sham)、缺氧缺血性脑损伤组(HIBD)、玛卡酰胺B处理组(Macamide B+HIBD),每组10只。采用Rice法建立新生小鼠HIBD模型,采用免疫荧光组织化学染色对ER α和ER β在新生小鼠HIBD脑内的细胞定位进行分析,Western Blot方法检测Macamide B对新生鼠HIBD脑内ER α和ER β表达的影响。结果:ER α和ER β在HIBD新生小鼠脑内神经元和星形胶质细胞中均有表达;与sham组相比,HIBD组ER α和ER β的表达量均明显降低;与HIBD组相比,Macamide B可显著上调ER α和ER β的表达。结论:Macamide B可通过上调新生小鼠脑内ER α和ER β表达表达水平对HIBD发挥神经保护作用。

Effects of Macamide B on the expression of estrogen receptor in brain of neonatal mice with hypoxic-ischemic brain damage

Objective: To observe the effects of macamide B on the expression of estrogen receptor α(ER α) and estrogen receptor β(ER β) in the brain of neonatal mice with hypoxic-ischemic brain damage(HIBD),and investigate the neuroprotective effect of Macamide B on HIBD newborn mice.Methods: Thirty C57BL/6 mouse pups at postnatal 7 day were used and divided into the following three groups: sham group(sham),HIBD group(HIBD),and HIBD pretreated with Macamide B group(Macamide B + HIBD).The HIBD model was established using a modified Rice method.Immunofluorescence histochemical staining was used to analyze the cellular localization of ER α and ER β in the brain of neonatal mice with HIBD;The effect of Macamide B on the expression of ER α and ER β in the brain of neonatal mice with HIBD was detected by Western Blot.Results: ER α and ER β were expressed in both neurons and astrocytes.The expression levels of ER α and ER β in HIBD group were significantly lower than those in sham group.Compared with the HIBD group,the expression levels of ERα and ER β in the Macamide B + HIBD group were significantly increased,and the difference was statistically significant.Conclusion: The neuroprotective effect of Macamide B on neonatal mouse HIBD may be related to the up-regulation of ER α and ER β expression in the brain.