武汉地区0～3 d新生儿末梢血网织红细胞相关参数参考区间

目的初步建立武汉市0~3 d新生儿末梢血网织红细胞(RET)6项参数的参考区间。方法选取2018年6—12月华中科技大学同济医学院附属同济医院产科病房0~3 d新生儿406名,检测其网织红细胞比例(RET%)、网织红细胞绝对值(RET#)、高荧光强度网织红细胞比例(HFR%)、中荧光强度网织红细胞比例(MFR%)、低荧光强度网织红细胞比例(LFR%)、网织红细胞血红蛋白含量(RET-He)共6项参数,用于建立生物参考区间,均采用x±1.96s表示。结果不同出生天数新生儿RET 6项参数差异均有统计学意义(P<0.05);除HFR%外,其他5项参数不同性别新生儿差异均无统计学意义(P>0.05);武汉地区0~3 d新生儿RET 6项参数的生物参考区间为:RET%第1天2.63%~6.27%、第2天2.55%~6.35%、第3天2.06%~6.06%;RET#第1天(0.139~0.397)×10^12/L、第2天(0.150~0.400)×10^12/L、第3天(0.124~0.386)×10^12/L;HFR%(男)第1天12.16%~30.97%、第2天7.64%~29.30%、第3天4.85%~20.64%;HFR%(女)第1天10.64%~27.67%、第2天6.78%~25.10%、第3天2.06%~19.26%;MFR%第1天13.48%~25.73%、第2天13.24%~26.61%、第3天11.45%~23.00%;LFR%第1天48.67%~71.07%、第2天48.24%~77.26%、第3天58.38%~83.56%;RET-He第1天29.58~37.30 pg、第2天29.25~36.23 pg、第3天27.49~34.85 pg;结论因人群所属地区和年龄不同,RET参考区间也会存在差异,本研究初步建立了武汉地区0~3 d健康新生儿RET 6项参数的参考区间,可为贫血性疾病的诊疗提供依据。

Reference intervals of peripheral blood reticulocyte and related parameters of 0-3 d neonates in Wuhan

Objective To establish the reference intervals of 6 items of peripheral blood reticulocyte(RET)and related parameters of 0-3 d neonates in Wuhan.Methods From June 2018 to December 2018,4060-3 d neonates in the Obstetrics Ward of Tongji Hospital of Huazhong Univerisity of Science and Technology Tongji Medical College were enrolled.A total of 6 items,including the percentage of reticulocyte(RET%),the absolute value of reticulocyte(RET#),high fluorescent ratio(HFR%),middle fluorescent ratio(MFR%),low fluorescent ratio(LFR%)and reticulocyte hemoglobin(RET-He),were determined for establishing the reference intervals.x±1.96s was used as biological reference interval Results The 6 items showed statistical significance between different days(P<0.05).Except for HFR%,the other 5 items showed no statistical significance between sex(P>0.05).The reference intervals of RET and related parameters of 0-3 d neonates in Wuhan were as follows.RET%:2.63%-6.27%on the 1st day,2.55%-6.35%on the 2nd day,and 2.06%-6.06%on the 3rd day.RET#:(0.139-0.397)×10^12/L on the 1st day,(0.150-0.400)×10^12/L on the 2nd day,and(0.124-0.386)×10^12/L on the 3rd day.HFR%(male):12.16%-30.97% on the 1st day,7.64%-29.30%on the 2nd day,and 4.85%-20.64% on the 3rd day;HFR%(female):10.64%-27.67% on the 1st day,6.78%-25.10%on the 2nd day,and 2.06%-19.26% on the 3rd day.MFR%:13.48%-25.73% on the 1st day,13.24%-26.61%on the 2nd day,and 11.45%-23.00% on the 3rd day.LFR%:48.67%-71.07% on the 1st day,48.24%-77.26% on the 2nd day,and 58.38%-83.56% on the 3rd day.RET-He:29.58-37.30 pg on the 1st day,29.25-36.23 pg on the 2nd day,and 27.49-34.85 pg on the 3rd day.Conclusions Due to different regions and ages,there are differences in the reference intervals of RET.The reference intervals of 6 items of RET and related parameters for 0-3 d neonates in Wuhan have established preliminarily,providing a reference for the diagnosis and treatment of anaemic diseases.