| CRISPR/Cas系统在分子检测中的应用 |
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| 引用本文： | 王雪亮,肖艳群,王华梁.CRISPR/Cas系统在分子检测中的应用[J].检验医学,2020,35(2):181-185. |
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| 作者姓名： | 王雪亮 肖艳群 王华梁 |
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| 作者单位： | 上海市临床检验中心分子生物学室, 上海 200126;上海市临床检验中心分子生物学室, 上海 200126;上海市临床检验中心分子生物学室, 上海 200126 |
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| 基金项目： | 国家重点研发计划(2017YFC0910000) |
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| 摘 要： | 近年来,成簇规律间隔短回文重复序列/成簇规律短回文重复序列相关蛋白(CRISPR/Cas)系统凭借其简单、高效的基因编辑能力,已被广泛应用于生物、医学等多个研究领域。随着CRISPR技术的快速发展,CRISPR/Cas系统已被开发为一种快速、便携、低成本、高灵敏度的分子检测工具,在病原体检测、耐药性分析、单核苷酸多态性(SNP)分型、肿瘤基因突变检测等方面取得重大突破。文章就不同Cas蛋白在分子检测中的最新研究进展进行综述,并对其应用前景进行展望,以期为从事相关领域的科研工作者提供参考与帮助。
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| 关 键 词： | 成簇规律间隔短回文重复序列 相关蛋白 附带切割 分子检测 诊断 |
Application of CRISPR/Cas system in molecular detection |
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| WANG Xueliang,XIAO Yanqun,WANG Hualiang.Application of CRISPR/Cas system in molecular detection[J].Laboratory Medicine,2020,35(2):181-185. |
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| Authors: | WANG Xueliang XIAO Yanqun WANG Hualiang |
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| Institution: | (Department of Molecular Biology,Shanghai Center for Clinical Laboratory,Shanghai 200126,China) |
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| Abstract: | In recent years,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPRassociated protein(Cas) system has been widely applied in biology,medicine and other research fields,because of its simple and efficient gene editing ability. With the rapid development of CRISPR technology,the CRISPR/Cas system has been developed as a rapid,portable,low-cost and high-sensitive molecular detection tool,and it has made great breakthroughs and progress in pathogen detection,drug resistance analysis,single nucleotide polymorphism(SNP) typing and tumor gene mutation detection. In order to provide a reference and help for researchers engaged in related fields,this review described the latest research progress of different Cas and their further application in molecular detection. |
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| Keywords: | Clustered regularly interspaced short palindromic repeat Clustered regularly interspaced short palindromic repeat-associated protein Collateral cleavage Molecular detection Diagnostics |
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