新型环烯烃聚合物微流控芯片的设计及其在大肠埃希菌O157:H7快速检测中的应用

目的建立一种可快速检测大肠埃希菌O157:H7的新型微流控芯片方法,用于细菌的早期鉴别与诊断。方法采用激光雕刻技术制作微流控芯片,在OCA光学透明胶上雕刻1条微通道,再将上、下2层环烯烃聚合物(COP)不可逆键合,最后通过免疫荧光方法检测大肠埃希菌O157:H7。比较微流控芯片法与培养法检测结果的总体符合率。结果成功建立了一种新型、简易的微流控芯片系统,对大肠埃希菌O157:H7的检测限为10~3CFU/mL,且有良好的特异性和可重复性,与培养法的总体符合率为95%。结论新型COP塑料芯片系统可实现对大肠埃希菌O157:H7的低成本、快速检测。

Role of COP microfluidic chip for the rapid detection of Escherichia coli O157:H7

Objective To establish a new type of plastic microfluidic chip which can detect Escherichia coli O157:H7 rapidly for the early diagnosis and differentiation. Methods The device contained microfluidic channels fabricated from cycloolefin polymer(COP) and optically clear adhesive(OCA). The microfluidic channels on OCA film were simply fabricated using a laser prototyping technique. Using immunofluorescence assay,Escherichia coli O157:H7 was detected. The consistency between microfluidic chip and culturing was evaluated. Results A new and simple microfluidic chip system had been established successfully. This system had a low detection limit of 10~3 CFU/mL,the repeatability and specificity were good,and the overall consistency with culturing was 95%. Conclusions This new type of COP plastic microfluidic chip system could detect Escherichia coli O157:H7 rapidly and inexpensively.