阿尔兹海默病患者炎性因子及CHE、Hcy水平的变化

目的探讨阿尔兹海默病(AD)患者外周血炎性因子C反应蛋白(CRP)、免疫球蛋白G(IgG)、中性粒细胞/淋巴细胞比值(NLR)]及胆碱酯酶(CHE)、同型半胱氨酸(Hcy)的变化。方法选取35例AD患者(AD组),以35例轻度认知障碍(MCI)患者(MCI组)作为疾病对照,以35名体检健康者作为正常对照。采用免疫比浊法检测CRP、IgG水平,采用硫代乙酰胆碱法检测CHE,采用循环酶法检测Hcy,采用流式细胞计数法检测中性粒细胞、淋巴细胞绝对值并计算NLR。结果与正常对照组比较,MCI组Hcy、CRP升高(P<0.05),CHE降低(P<0.05),IgG及NLR差异均无统计学意义(P>0.05);AD组IgG、Hcy、CRP、NLR升高(P<0.05),CHE降低(P<0.05)。与MCI组比较,AD组IgG、Hcy和NLR升高(P<0.05),CHE降低(P<0.05),CRP差异无统计学意义(P>0.05)。相关性分析结果显示,AD组Hcy与NLR呈负相关(r=-0.344,P=0.043),其他项目之间均无相关性(P>0.05)。结论CRP、IgG、CHE、Hcy、NLR可能在AD的诊断及与MCI的鉴别诊断中有一定的价值。

Changes of inflammatory factors,CHE and Hcy levels in patients with Alzheimer's disease

Objective To observe the changes of inflammatory factorsC-reactive protein(CRP),immunoglobulin G(IgG),neutrophil/lymphocyte ratio(NLR)],cholinesterase(CHE)and homocysteine(Hcy)in peripheral blood of patients with Alzheimer's disease(AD).Methods A total of 35 AD patients(AD group)and 35 patients with mild cognitive impairment(MCI)(MCI group,disease control group)were enrolled,and 35 healthy subjects were enrolled as healthy control group.CRP and IgG were determined by immunoturbidimetry,the determination of CHE was performed by thio-acetylcholine method,and the determination of Hcy was performed by circulatory enzyme method.The absolute values of neutrophils and lymphocytes were determined by flow cytometry,and the NLR was calculated.Results Hcy and CRP in MCI group were higher than those in healthy control group(P<0.05),and CHE decreased(P<0.05).There was no statistical significance of IgG and NLR(P>0.05).IgG,Hcy,CRP and NLR increased in AD group(P<0.05),and CHE decreased(P<0.05).Compared with MCI group,IgG,Hcy and NLR increased in AD group(P<0.05),and CHE decreased(P<0.05).There was no statistical significance in CRP(P>0.05).Hcy and NLR were negatively correlated(r=-0.344,P=0.043).There was no correlation among the other indicators in AD group(P>0.05).Conclusions CRP,IgG,CHE,Hcy,NLR may be of value in the diagnosis of AD and differential diagnosis of MCI.