目测法APTT混合纠正试验的鉴别能力评价

目的采用目测法进行活化部分凝血活酶时间(APTT)混合纠正试验,并评价其对凝血因子缺陷与凝血因子抑制剂的鉴别能力。方法收集APTT延长患者标本62例,其中凝血因子Ⅷ抑制剂阳性标本22例,内源性凝血因子缺陷标本20例,狼疮抗凝物(LA)阳性标本20例。分别采用Rosner指数法与目测法APTT混合纠正试验对标本进行检测。结果 Rosner指数法与目测法APTT混合纠正试验判断凝血因子缺陷标本的敏感性均为100.0%,判断LA的敏感性均为95.0%。50%点差值法与目测法APTT混合纠正试验判断凝血因子抑制剂的敏感性均为95.5%。结论目测法APTT混合纠正试验对凝血因子缺陷和凝血因子抑制剂的鉴别能力与Rosner指数法相当,且试验操作、结果判读更为简便。

Evaluation on the differential ability of visual APTT mixed correction test

Objective To evaluate the differential ability of visual activated partial thromboplastin time(APTT)mixed correction test for coagulation factor deficiency and coagulation inhibitor.Methods A total of 62 specimens with prolonging APTT were collected,including 20 cases of coagulation factor deficiency,22 cases of coagulation inhibitorⅧpositive and 20 cases of lupus anticoagulant(LA)positive.Visual APTT mixed correction test and Rosner index method were used.Results The sensitivities of Rosner index method and visual APTT mixed correction test for judging coagulation factor deficiency were 100.0%,and the sensitivities for judging LA were 95.0%.The sensitivities for judging coagulation inhibitor were 95.5%by visual APTT mixed correction test and 50%difference method.Conclusions Using the visual APTT mixed correction test,the differential ability of coagulation factor deficiency and coagulation inhibitor is comparable to Rosner index method,and visual APTT mixed correction test is feasible for interpreting results.