室内挥发性有机物诱发大鼠皮肤风团形成的研究

目的:研究室内挥发性有机物对大鼠皮肤风团形成的机制。方法:以36只Wistar大鼠为研究对象,采用大气采样机采取混有VOC的空气样本,作用于大鼠(VOCL组),3个月后再取变应原液注射于大鼠,观察皮肤及胸腺组织改变。并与双蒸水组、短期接触VOC组(VOCS组)对照。结果:VOCL组皮肤真皮浅层水肿,可见炎性细胞浸润,少数嗜酸粒细胞。胸腺组织CD4 、CD8 细胞表达减少,CD8 的百分比较CD4 的百分比高;双蒸水组和VOCS组炎性细胞较少;VOCS组CD4 ,CD8 表达细胞增多。结论:VOC低水平长时间作用可导致机体的超敏反应,有可能使变应原诱发慢性荨麻疹。

Experimental study for the pathogenesis of chronic urticaria induced by volatile organic compounds in rat

Objective:To investigate the pathogenesis of chronic urticaria induced by volatile organic compounds (VOC) in rat. Methods: Thirty-six Wistar rats were divided into three groups: VOCL group, control (double distilled water) group and short VOC group (VOCS). Indoor air with VOC was collected with atmosphere sampling-machine, and then injected into Wistar rats with allergens 3 months later. Histopathology of skin and thymus tissue was examined. Results: In VOCL group the pathological picture demonstrated that there were edematous in dermis and more inflammatory infiltration with eosinophilic granulocytes than in control and VOCS groups. The infiltration of CD4 and CD8 was decreased and the expression of CD8 cells was more evidenced than CD4 cells in thymus tissue. In control and VOCS groups there was less inflammatory infiltration and more expression of CD4 and CD8 cells.Conclusion: Low level of VOC can induce the hypersensitivity reaction, which evokes chronic urticaria through inhibiting immune function.