大豆磷脂对小鼠学习记忆及脑组织脂类成分的影响

目的　大豆磷脂对小鼠学习记忆、脑组织蛋白质和脂类成分的影响。方法　小鼠用不同剂量的大豆磷脂灌胃 ,分别为低剂量组 (2 5g kg)、中剂量组 (5 0g kg)、高剂量组 (10 0g kg) ,同时设立对照组 (去离子水 ) ,连续灌胃 30天 ,进行小鼠学习记忆测定后处死 ,取全脑 ,测定小鼠脑组织中蛋白质含量、磷脂和脂肪酸的成分及含量。结果　 (1)与对照组比较 ,高、中剂量组小鼠学习记忆能力显著增强 (P <0 0 5 ) ;低剂量组小鼠记忆力显著改善 (P <0 0 5 )。 (2 )各剂量组脑重占体重的比例及脑组织中蛋白质含量均显著上升 (P <0 0 5 )。 (3)高剂量组C1 8:2 含量、C1 8:3含量、PUFA占总脂肪酸的比例显著提高 (P <0 0 5 ) ;中剂量组C1 8:3含量有显著差异 (P <0 0 5 )。 (4 )高剂量组PS、PE、PC、PL含量显著增加 (P <0 0 5 ) ,SM PC的比值则显著下降 (P <0 0 5 ) ;中剂量组PS、PC、PL含量显著增加 (P <0 0 5 )。结论　提示大豆磷脂可明显提高小鼠脑组织中蛋白质、PUFA和PL的含量 ,显著改善小鼠学习记忆能力 ,且与大豆磷脂存在剂量对应关系。

Effects of soybean phospholipids on learning and memory ability and contents of lipids in mice's brain

Objective To study the effects of soybean phospholipids on learning and memory abilities and contents of protein and lipids in brain of mice.Methods Mice were randomly divided into four groups: low dose (2.5g/kg), middle dose(5.0g/kg) and high dose (10.0g/kg) of soybean Phospholipids in diet and control group (distilled water). The mice were fed with soybean phospholipids everyday while control groups were fed with distilled water for 30days. After learning and memory abilities were measured, the mice were killed and contents of protein, fatty acids and phospholipids in brain of mice were determined. Results The learning and memory abilities of mice fed with middle and high dose of soybean Phospholipids were improved obviously(P<0.05), while memory abilities of low dose's mice were stronger than that of control(P<0.05).(2) The brain weight v.s body weight of those in three doses' group was obviously increased, and the protein content in brain was significantly higher compared with control(P<0.05).(3)The contents of linoleic acid(C 18:2), linolenic acid(C 18:3) and ratio of polyunsaturated fatty acids(PUFA) to total fatty acids(TFA) obviously increased(P<0.05). In brain of high dose mice, there was obvious change in the content of linolenic acid(C 18:3) in middle's (P<0.05).(4) The contents of phosphatidylseline(PS) phosphatidylethanolamine( PE) , Phosphatidylcholine(PC) and phospholipids (PL) obviously increase(P<0.05), while ratio of SM to PC was decreased significantly in brain of high dose mice(P<0.05). The contents of PS, PC and PL were increased obviously(P<0.05). Conclusion Soybean phospholipids may increase contents of protein, PUFA and PL in brain of mice and improve learning and memory abilities of mice effectively in a dose-response manner.