P27蛋白在人脑胶质瘤组织中的表达及其临床意义(英文)

目的探讨 P27在人脑胶质瘤组织中的表达及其应用价值。方法采用 S-P 免疫组化技术对48例脑胶质瘤患者按 WHO 分类标准的不同恶性程度,将人脑胶质瘤组织和12例非肿瘤脑组织中 P27蛋白的表达作了检测,并用彩色图像分析系统对染色结果进行定量分析,同时将其与患者临床特点密切联系进行对比,最后得出结论。结果 P27蛋白免疫反应复合物定位于细胞核,并在人脑胶质瘤组织中的表达明显较非肿瘤组织为高,且 P27蛋白的表达程度随肿瘤恶性程度的增高而下降,同时 P27蛋白的表达强度愈低则患者预后就越差。结论 P27蛋白的异常表达可能与人脑胶质瘤的发生和发展密切相关,其表达强度可客观地反映肿瘤的恶性程度和评价预后。

P27/Kipl Expression in Gliomas and Its Clinical Significance

Objective:To study p27/Kip1 expression in gliomas and its application value.Methods:Im- munohistochemical technique was used to detect the exprssion of p27/Kip1 gene in 48 different malignant grade human brain glioma tissues categorized according to WHO classification and 12 normal human brain tissues,which were analyzed quantitatively by using the image system and compared retrospectively with the patients clinical characteristics.Results:In this series,the immunohistochemical reaction for p27/Kip1 was confined to the nuclei.The abnormal positive expression rate of p27/Kip1 in gliomas was found to be higher than that in normal tissues(P<0.05).The positive nuclei expression of p27/Kip1 de- creased in number and staining intensity with the increasing degree of histological malignancy(P<0.05). Lower expression of p27/Kip1 was associated with poor prognosis(P<0.05).P27/Kip1 expression could be regarded as an independent prognostic factor.Conclusion:The abnormal expression of p27/Kip1 may be closely related to the occurrence and development of gliomas,and also can be used to evaluate the prognosis independently.