刺五加皂甙B对心肌线粒体ATP敏感性钾通道的作用

研究刺五加皂甙B(Sb)对心肌线粒体ATP敏感性钾通道 (mitoKATP)的作用。用酶解法获取兔心室肌细胞 ,分为对照组、0 .1mmol/LSb组、0 .5mmol/LSb组、1mmol/LSb组、格列本脲组和格列本脲 +1mmol/LSb组。用罗丹明 (Rhodamine 12 3)染色 ,激光扫描共聚焦显微镜 (多光子模式 )观察线粒体荧光强度变化。结果 :①观察 15min对照组线粒体荧光强度无明显变化。② 0 .1,0 .5和 1mmol/LSb组均可见用药后线粒体荧光强度明显增加 ,分别增加 10 .3%± 6 .78%、18.4 %± 5 .5 1%和 2 4 .1%± 7.6 4 % ,荧光强度的增加以前 5min为主。③ 3μmol/L格列本脲不影响线粒体荧光强度 ,但可以阻断Sb对线粒体荧光强度的作用。结论 :Sb对mitoKATP有开放作用。

The Effect of Acanthopanax Senticosides B on Myocardial Mitochondrial ATP Sensitive Potassium Channel

The effect of Acanthopanax senticosides B (Sb) on myocardial mitochondrial ATP sensitive potassium channel (mito K ATP ) was studied . Ventricular myocytes of rabbits were isolated by enzymatic dissociation and divided into control group,0.1mmol/L Sb group,0.5mmol/L Sb group,1mmol/L Sb group,Glibenclamide group,and Glibenclamide +1mmol/L Sb group. Rhodamine 123 was choosed to stain the mitochondria. Laser scanning confocal microscope (multi photon) was used to observe the variation of mitochondrial fluorescence intensity in different groups for 15 min. Results: ①There was not obvious variation of mitochondrial fluorescence intensity in control group during 15 min observation. ②Mitochondrial fluorescence intensity increased significantly in 0.1mmol/L Sb group,0.5mmol/L Sb group, and 1mmol/L Sb group after treatment. The increments were 10.3%±6.78%,18.4%±5.51% and 24.1%±7.64%. The increment of mitochondrial fluorescence intensity was mainly in the first five minutes. ③3μmol/L Glibenclamide had no effect on mitochondrial fluorescence intensity and could block the effect of Sb on mitochondrial fluorescence intensity. Conclusion: Sb could open the mitoK ATP .