荧光光度法测定竹叶柴胡地上部分不同部位总黄酮含量

目的：分析竹叶柴胡不同部位总黄酮含量，研究黄酮在柴胡不同部位的分布，为中药资源合理开发利用提供参考。方法以芦丁为对照品，以5%硝酸铝与样品中黄酮成分产生络合反应，选择激发波长λex=445 nm，发射波长λem=484 nm，应用荧光光度法测样品的荧光光度值，计算总黄酮含量。结果以芦丁在0.198~3.168μg/mL范围内与荧光强度线性关系良好，y=0.9728x-0.001，r2=0.9993。竹叶柴胡花、叶、茎中总黄酮含量最分别为3.08%、1.85%、0.41%。结论竹叶柴胡花、叶中总黄酮含量较高，应在开花期采收竹叶柴胡地上部分。

Determination of total flavonoids in different aerial parts of Bupleurum marginatum by fluorescence spectrophotometry

Objective To establish a method to determine the total flavonoid levels in flower, leaves and stalk of Bupleurum marginatum by Spectrofluorimetry, and to provided a reference for reasonable development and utilization of traditional Chinese medicine resources. Methods Based on fluorescent property that flavonoids could make a stable complexation with Al3+, and the excitation and emission wavelengths were selected at 445 nm and 484 nm respectively with rutin as the standard sample. The total flavonoids levels were determined in the sample by fluorescence spectropho-tometry. Results The fluorescence intensity had a good linear relationship with rutin concentration in the range of 0.198~3.168μg/mL. The regression equation was found to be y=0.972 8x-0.001, r2=0.999 3. The content of flavonoids in flower, leaves and stalk were 3.08%, 1.85%, 0.41%, respectively. Conclusion The flower, leaves of Bupleurum mar-ginatum contain relatively higher levels of total flavonoids, which provides a reference for harvesting the aerial parts of Bupleurum marginatum in flowering period.