| IVIG对新生儿脐带血单核吞噬细胞表面分子的影响 |
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| 引用本文： | 王美红,陈同辛,朱亚忠,李庆生. IVIG对新生儿脐带血单核吞噬细胞表面分子的影响[J]. 现代免疫学, 2006, 26(1): 56-59 |
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| 作者姓名： | 王美红 陈同辛 朱亚忠 李庆生 |
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| 作者单位： | 上海交通大学附属新华医院,上海市儿科医学研究所免疫/肿瘤研究室,上海,200092 |
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| 基金项目： | 中国科学院资助项目;上海市高等教育科技发展基金 |
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| 摘 要： | 为从脐带血单核吞噬细胞(Mo/Mφ)表面CD14、HLA-DR和CD86分子表达的角度来探讨IVIG对新生儿免疫抑制作用,将IVIG、GM-CSF和LPS不同组合对Mo/Mφ进行刺激培养,利用双色免疫荧光抗体标记-流式细胞技术检测Mo/Mφ表面CD14、HLA-DR和CD86分子的表达。加入IVIG处理培养的脐带血Mo/Mφ表面CD14、HLA-DR和CD86分子的阳性细胞比率和相对荧光密度均明显降低,同时CD14+HLA-DR+和CD14+CD86+细胞的比率明显降低。IVIG可通过抑制脐带血Mo/Mφ表面CD14、HLA-DR和CD86分子的表达而抑制其抗原提呈能力,这可能是IVIG免疫抑制的机理之一。
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| 关 键 词： | 新生儿 静脉免疫球蛋白 单核吞噬细胞(Mo/Mφ) CD14 HLA-DR CD86 |
| 文章编号： | 1001-2478（2006）01-0056-04 |
| 收稿时间： | 2004-06-03 |
| 修稿时间： | 2004-09-07 |
Effects of intravenous immunoglobulin upon the surface molecules on the neonatal umbilical cord blood monocytes/macropahages |
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| WANG Mei-hong,CHEN Tong-xin,ZHU Ya-zhong,LI Qing-sheng. Effects of intravenous immunoglobulin upon the surface molecules on the neonatal umbilical cord blood monocytes/macropahages[J]. Current Immunology, 2006, 26(1): 56-59 |
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| Authors: | WANG Mei-hong CHEN Tong-xin ZHU Ya-zhong LI Qing-sheng |
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| Affiliation: | Immunology/Oncology Depart ment , Shanghai Institute for Pediatric Reseach , Affiliated Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, China |
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| Abstract: | In order to investigate the inhibitory mechanism of intravenous immunoglobulin(IVIG) upon the surface molecules(CD14,HLA-DR,CD86) on neonatal umbilical cord blood monocytes/macrophages(Mo/Mφ),this kind of Mo/Mφ was stimulated with different combinations of IVIG,GM-CSF and LPS,and the expressions of CD14,HLA-DR and CD86 on Mo/Mφ were detected by means of dual-color immunofluorescence antibody staining and flow cytometry(FCM).In addition,the results were compared with those of the adults.It was found that the per-centages of CD14 +,HLA-DR + and CD86 + cells and the relative intensity of fluorescence were significantly re-duced when the Mo/Mφ were treated with IVIG.Also,IVIG could inhibit the activation of the Mo/Mφ induced by GM-CSF and LPS.It is concluded that IVIG can suppress the capacity of antigen presentation of umbilical cord blood Mo/Mφ,and this may be the mechanism for the inhibitory effect of intravenous immunoglobulin. |
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| Keywords: | neonates intravenous immunoglobulin monocytes/macrophages CD14 HLA-DR CD86 |
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