强脉冲光对皮肤热休克蛋白70表达的影响

目的观察强脉冲光对皮肤热休克蛋白70表达的影响，探讨光子嫩肤的分子生物学机制方法选择15只SD大鼠，每只选3个部位用强脉冲光照射，采用同一照射参数，能量密度34J／cm^2，分为3个脉冲，脉宽各为4、5、6ms，脉冲延时为20及25ms照射后第1、3、5、7、15、30天分别于治疗及非治疗部位切取皮肤样本，免疫组化染色，观察：结果照射部位第1天皮肤表皮、皮脂腺细胞、毛细血管内皮细胞均呈阳性染色，第7天达高峰，第15天染色渐弱，第30天染色基本消失．非治疗部位染色阴性。结论强脉冲光照射皮肤后可引起热休克蛋白70表达的增加，提示热休克蛋白70在光子嫩肤过程中起一定作用。

Effect of intense pulsed light on heat shock protein 70 expression in skin]

OBJECTIVE: To observe the effect of intense pulsed light (IPL) on heat shock protein 70 (HSP70) expression in skin and elucidate the biological mechanisms of photorejuvenation. METHODS: The skin of 15 SD rats was exposed to IPL in 3 regions at the energy density of 34 J/cm(2) in triple pulses with the duration of 4, 5 and 6 ms, respectively, and pulse delays of 20 and 25 ms. On days 1, 3, 5, 7, 15 and 30 after treatment, specimens of the treated and untreated skin were taken, respectively, for determination of HSP70 expression by immunohistochemistry. RESULTS: In treated regions, positive immunohistochemical staining was observed on day 1 in the epidermal keratinocytes, sebaceous gland cells and endothelial cells. The staining reached the highest intensity on day 7, gradually weakened on day 15, and disappeared on day 30. In the untreated areas, the cells were negative for immunohistochemical staining. CONCLUSION: Skin HSP70 expression can be enhanced by IPL, suggesting the role of HSP70 in photorejuvenation.