| p38MAPK通路在健骨颗粒促成骨细胞早期分化中的作用 |
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| 引用本文： | 吴银生,林煜,卢天祥,郑良朴,陈旭征,林燕萍.p38MAPK通路在健骨颗粒促成骨细胞早期分化中的作用[J].福建中医学院学报,2010,20(6). |
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| 作者姓名： | 吴银生 林煜 卢天祥 郑良朴 陈旭征 林燕萍 |
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| 作者单位： | 1. 福建中医药大学中西医结合研究院,福建,福州,350108
2. 福建省泉州市第一医院骨科,福建,泉州,362000 |
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| 基金项目： | 教育部博士点基金项目，福建省科技厅重点项目，福建省高校中西医结合基础重点实验室开放课题基金资助项目·2008年度陈可冀中西医结合发展基金 |
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| 摘 要： | 目的观察p38MAPK信号转导通路在健骨颗粒促进体外培养成骨细胞早期分化过程中的作用。方法采用酶消化法分离、培养SD大鼠成骨细胞,分4组进行干预:对照组(加入生理盐水血清)、阻断剂组(加入p38MAPK的阻断剂SB202190)、中药组(加入健骨颗粒含药血清)和中药加阻断剂组(加入健骨颗粒含药血清和SB202190),运用化学比色法检测上清液中碱性磷酸酶(AKP)活性及羟脯氨酸(HYP)含量,实时荧光定量PCR-SYBR GREEN法检测成骨细胞Ⅰ型胶原(ColⅠ)的表达。结果在AKP活性、HYP含量及ColⅠmRNA表达3项指标上,中药组>中药加阻断剂组>对照组>阻断剂组,各组间差异有统计学意义(P<0.05)。结论体外培养大鼠成骨细胞的早期分化与p38MAPK信号转导通路有关;健骨颗粒含药血清能促进成骨细胞的早期分化,但这一作用仅部分通过p38MAPK信号通路实现。
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| 关 键 词： | 成骨细胞 细胞分化 血清学试验 中药疗法 |
Effect of p38MAPK Pathway on Early Differentiation of Osteoblasts Induced by Jiangu Granula |
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| WU Yin-sheng,LIN Yu,LU Tian-xiang,et al.Effect of p38MAPK Pathway on Early Differentiation of Osteoblasts Induced by Jiangu Granula[J].Journal of Fujian College of Traditional Chinese Medicine,2010,20(6). |
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| Authors: | WU Yin-sheng LIN Yu LU Tian-xiang |
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| Institution: | WU Yin-sheng1,LIN Yu1,LU Tian-xiang2,et al(1.Fujian Academy of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China,2.Department of Orthopaedics,Quanzhou First Hospital,Quanzhou,Fujian 362000,China) |
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| Abstract: | Objective To investigate the effect of p38MAPK pathway on early differentiation of osteoblasts(OB) in vitro induced by Jiangu granula(JGG).Methods Primary cells were isolated from calvaria of newborn SD rats and performed serial subcultivation by enzymatic digestion.The cells were divided into 4 group,the control group was interfered with serum containing normal saline,SB group with SB202190,JGG group with serum containing JGG,and JGG+SB group with both serum containing JGG and SB202190.The activity of alka... |
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| Keywords: | p38MAPK |
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