Quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside metabolites in the rat using UPLC-Q-TOF/MS |
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作者姓名: | YAO Xin ;ZHOU Gui-Sheng ;TANG Yu-Ping ;SHANG Er-Xin ;GUO Jian-Ming ;QIAN Da-Wei ;DUAN Jin-Ao |
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作者单位: | [1]Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory forHigh Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese MedicinalResources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; [2]Department of Pharmacy, First Affiliated Hospital of Sooehow University, Suzhou 215006, China |
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基金项目: | This project was supported by the National Science and Technology Support Program of China (No. 2011 BAI04B03). |
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摘 要: | Ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS) and the MetabolynxTM software, combined with mass defect filtering, were applied to identity the metabolites of quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside(QGR) in rats after intravenous administration. MSE was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the rapid structural characterization of eight metabolites in rat plasma, urine and bile. The results indicated that methylation and glucuronidation were the major metabolic pathways of QGR in vivo. The present study provided important information about the metabolism of QGR which will be useful for fully understanding the mechanism of action of this compound. Furthermore, this work demonstrated the potential of the UPLC-Q-TOF/MS approach using Metabolynx for rapid and automated research of the metabolites of natural products.
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关 键 词: | glucopyranosyl Quercetin-3-O quercetin methylation intravenous administration collision simultaneous acetonitrile automated |
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