人牙本质磷蛋白的提取与特性分析

目的 建立人牙本质磷蛋白的提取方法并分析其特性。方法，将人牙本质粉用０．６ｍｏｌ／Ｌ ＨＣｌ脱矿后，用ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ ＣＬ６Ｂ离子交换层析柱分离纯化ＤＰＰ，取纯化的ＤＰＰ，用紫外分光光度计检测其吸光度值；用等离子发射光谱分析其磷含量；ＳＤＳ－ＰＡＧＥ测量相对分子质量；并在水解后进行氨基酸含量分析。结果 用０．６ｍｏｌ／Ｌ ＨＣｌ脱矿，可以有效地提取人ＤＰＰ。所提取的ＤＰＰ是磷含量较高

The isolation and characterization of dentin phosphoprotein from human dentine]

OBJECTIVE: To establish the method of isolation and characterization of dentin phosphoprotein (DPP) from human dentine. METHODS: Dentin powder was placed in dialysis tube after demineralized to completion with 0.6 mol/L HCl, then isolated DPP through DEAE-Sepharose CL6B anion exchange chromatography. Its protein composition, organic phosphorus content, molecular weight and amino acid were analyzed. RESULTS: DPP contained high organic phosphorus, 23.8% of aspartic residue and 19.1% serine residue; their molecular weight were 141, 000, 124, 000, 108, 000 respectively. CONCLUSION: DPP can be extracted from human dentine with 0.6 mol/L HCl demineralization. and DEAE-Sepharose CL6B anion exchange chromatography. It is a group of proteins which have a high content of organic phosphorus and is found to be rich in serine and aspartic acid residues.