杏仁对吸烟导致DNA氧化损伤的保护作用

背景与目的： 研究美国加州杏仁对吸烟诱导的DNA氧化损伤是否具有保护作用。材料与方法： 选取30个吸烟的志愿者，随机分3组（每组10人），每天分别给予0， 84和168 g的杏仁，共28 d；试验期间各组对象膳食和吸烟量相同，在实验开始前和试验结束后分别收集新鲜尿液测定柯替宁，采静脉血做彗星实验，收集24 h尿测定8-OH-dG。结果： 彗星实验显示，168 g/d剂量组DNA损伤程度比对照组和试验前均显著降低(P<0.05)；8-OH-dG 测定结果显示，84 g / d和168 g / d剂量组的8-OH-dG水平明显低于试验前和对照组(P<0.05)。结论： 食用美国加州杏仁可能具有降低吸烟人群DNA氧化损伤的作用。

The Protective Effect of Almond on DNA Damage Induced by Cigarette Smoke

BACKGROUND & AIM: Exposure of cigarette smoke (CS) can increase oxidative damage, this research aimed to study the protective effect of supplementation of almond on DNA damage in smokers. MATERIAL AND METHODS: Thirty smokers were recruited into our study, and were divided into three groups(n=10) which received almond 0 g/d, 84 g/d and 168 g/d respectively, and the study period was for 28 days. In the beginning and the end of the study, cotinine and 8-hydroxy- 2'deoxyguanosine (8-OH-dG) levels in urine were analysed, and comet assay of peripheral blood lymphocytes was conducted. RESULTS: In the end of the study , compared with control group and baseline, a significant decrease of oliveive tail moment and tail/head length in comet assay in 168 g/d group was observed (P<0.05). The 8-OH-dG level decreased significantly in 84 g/d and 168 g/d groups(P<0.05). CONCLUSION: The results suggested that supplementation of almond might reduce DNA damage in smokers.