Balb/c小鼠小肠Cajal间质细胞形态学特征及慢波活动的研究

目的 观察未成年Balb/c小鼠小肠Cajal间质细胞(ICC)的形态及分布,并测定其慢波活动情况,以进一步认识ICC的发育.方法 首先,通过免疫组织化学方法观察10天大小Balb/c小鼠小肠ICC的分布及形态特征,采用电镜观察ICC的超微结构;再分别测定10d大小Balb/c小鼠及成年Balb/c小鼠小肠在体慢波活动情况.结果 ICC主要分布于小肠环肌层与纵肌层间区(称之为ICC-MY),以及环形肌的内薄层与外厚层间(称之为ICC-DMP).ICC-MY呈现为连续性分布特点.电镜结果显示ICC有2个以上长突起,胞浆内含有大量的线粒体,而中间丝很少见.成年小鼠小肠慢波活动表现为近似正弦波样曲线,频率为(17.78±1.52)次/min,振幅为(0.16±0.02)mV,10 d大小Balb/c小鼠小肠慢波活动类似于成年小鼠,但其频率为(14.00±0.98)次/分,振幅为(0.09±0.02)mV,均明显低于成年小鼠(P＜0.01).结论 Balb/c小鼠小肠主要分布着ICC-MY及ICC-DMP两类ICC,为双极或多极细胞,ICC-MY相互连接成网络状;未完全发育好的ICC表现出不成熟的慢波活动.

Study on morphological feature of interstitial cells of Cajal and slow waves in Balb/c murine small intestine

[Objective] To observe the distribution and morphology of interstitial cells of Cajal (ICC) in young Balb/c murine small intestine and determine the activities of slow waves, and to recognize the development of ICC more clearly. [Methods] Firstly, the distribution and configuration of ICC were observed with immunohistochemical method and the ultrastructure of ICC was observed by an electron microscope in the small intestine of 10-days postpartum Balb/c murine. Secondly, the slow waves of small intestine in vivo were measured in 10-days postpartum Balb/c murine and in adult Balb/c murine. [Results] ICC were located mainly between the circular and longitudinal muscle layers (called ICC-MY) and between the internal thin layer and the thick outer layer of the circular muscle(called ICC-DMP) in the small intestine. The distribution of ICC-MY was continuous. Electron microscope showed that ICC had two or more very long main processes and contained abundant mitochondria, while intermediate filaments were rarely seen. The slow waves of small intestine were proximal cosinal waves in the adult Balb/c murine and the frequency was (17.78±1.52) cycles/min and the amplitude was (0.16±0.02) mV. The slow waves in the 10-days postpartum Balb/c murine resembled those in adult murine and the frequency was (14.00±0.98) cycles/min and the amplitude was (0.09±0.02) mV (P＜0.01 vs the adult murine). [Conclusion] Two types of ICC including ICC-MY and ICC-DMP are observed in the young Balb/c murine small intestine which are bipolar or multipolar cells.ICC-MY interconnects with a network. ICC that are undeveloped fully has immature slow waves.