二甲基苯并蒽诱导地鼠颊癌过程中Rb基因产物表达的研究

目的探讨实验性吕必生过程中Ｒｂ基因编码产物（ｐＲｂ）的表达变化。方法 用０．５％的二甲基苏醒 并蒽（ｄｉｍｅｔｈｙｌ－ｂｅｎｚａｎｔｈｒａｃｅｎｅ，ＤＭＢＡ）丙酮液涂技法 鼠石侧颊粘膜３次／周，对病变组织用ＬＳＡＢ法进行免疫组化染色（ｐＲｂ染色最强，３周（单纯增生）尖似正常；６、９周异常增生损害时染色减弱，与异常增生程度有关；１２周 形成时染色进一步减弱。ｐＲｂ染色降低与恶变进展有关。结论 ＤＭＢ

The abnormal expression of pRb during DMBA inducde hamster buccal pouch carcinogenesis

Objective To study the expression and role of pRb in 7,12 dimethylbenzanthracene(DMBA) induced hamster buccal pouch carcinogenesis. Methods\ DMBA (0.5%) in acetone was applied to the right buccal pouch 3 times per week for up to 12 week. Paraffin embedded sections were used for pRb immunohistochemical determination (LSAB technipue,polyclonal antibody c 15 for pRb), The density of staining was analysed by rank sum test.Results\ The positive staining of pRb was strong in normal buccal epithelium, noalteration of pRb staining in 3 week (hyperplastic epithelium) and somewhat reduced between 6 and 9 week (dysplastic lesion),but it was marked reduced in 12 week (carcinoma). Statistical analysis found that changes of pRb were associated with stages of carcinogenesis. Conclusion\ These results indicate that abnomal expression of Rb gene product could be related to oral carcinogenesis and may serve as a biomarker for supervising oral malignancy.