大鼠弥漫性轴索损伤后丘脑NGBmRNA的表达

目的研究弥漫性轴索损伤后大鼠丘脑组织中脑红蛋白（NGB）mRNA的表达变化，初步探讨NGB与颅脑损伤的关系。方法选择改进的Mamarou法制作弥漫性轴索损伤模型，采用RT-PCR检测颅脑损伤后不同时间丘脑组织中NGBmRNA的表达情况，并对所得数据进行分析。结果在伤后30min脑组织中NGBmRNA的表达明显升高，而24h时则有所下降，48h又上升达高峰。结论弥漫性颅脑损伤后，丘脑组织中NGBmRNA表达升高，提示其可能参与神经元损伤后应激及继发缺血、缺氧性脑损伤的应答机制。

mRNA expression of neuroglobin in thalamus of DAI rats

Objective To study mRNA expression of neuroglobin (NGB) in thalamus of DAI rats and explore the relation between NGB and heed injury. Method 46 male SD rats were randomly divided into four groups. The DAI model was established according to modi-fied Mamamu method. The mRNA expression of NGB in thalamus at different time point after injury was detected by RT-PCR method. All data were analyzed with statistical software. Result The mRNA expression of NGB in thalamus increased obviously from 30 minutes after in-jury while decreased at the 24th hour after injury. But the expression increased again and reached peak at the 48th hour after injury. Conclu-sion The mRNA expression of NGB in thalamus increased after DM. Those changes suggested NGB might participate in the response mech-anism of stress, secondary ischemia and hypoxic brain injury after neuron injury.