广西北部乙型肝炎病毒基因型流行分布的研究

目的　了解广西北部桂林乙型肝炎病毒 ( HBV)基因型的流行分布情况。方法　用套式 PCR扩增检测 41例桂林慢性无症状 HBV携带者血清 HBV DNA,阳性者用直接测序法进行 HBV基因分型。结果　 1 9例 HBV DNA阳性 ,阳性率为 46.34% ( 1 9/ 4 1 ) ,其中 1 5例为基因型 B,占 78.9% ( 1 5 / 1 9) ,4例为基因型 C,占 2 1 .1 % ( 4 / 1 9) ,未见到其它目前已知的 HBV基因型 A、D、E、F、G和 H;另外 ,还发现 HBV基因型 C组 HBe Ag阳性率显著高于基因型 B组 ( P<0 .0 5 ) ,提示 HBV基因型 C比 HBV基因型 B危害更大。结论　广西北部桂林流行的 HBV基因型有基因型 B和 C两种 ,以基因型 B为主要流行的优势基因型。

Distribution of hepatitis B virus genotypes in Northern of Guangxi

Objective To understand the distribution of hepatitis B virus genotypes in GuiLin,Northern Guangxi.Methods The nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA in sera of 41 asymptomatic carriers(ASC) from GuiLin. The genotypes of the positive were determined by direct sequencing.Results Nineteen of them were positive for HBV DNA. Positive rate was 46.34%(19/41).15 cases'genotype was genotype B of HBV, counting for 78.9%(15/19). Four cases' was genotype C of HBV, counting for 21.1%(4/19). No other genotypes(such as genotype E,F,G,H of HBV) were found. The HBeAg positive rate was higher in the carriers with genotype C of HBV than in that with genotype B of HBV (P<0.05).Conclusions HBV genotypes in GuiLin,Northern Guangxi is genotype B and C of HBV. The major genotype is genotype B of HBV in GuiLin,Northern Guangxi.