活血荣络片对大脑中动脉缺血模型大鼠脑组织微囊蛋白-1表达的影响

目的观察活血荣络片对大脑中动脉缺血(MCAO)模型大鼠脑组织中细胞膜微囊蛋白-1(Caveolin-1)表达的影响,探讨活血荣络片对脑缺血后神经功能修复的作用机制。方法将96只SD大鼠随机分为假手术组(24只)和造模组(72只),造模组采用线栓法制作MCAO模型,造模成功后再随机分为模型组、对照组及实验组,各24只。假手术组及模型组予蒸馏水2 m L灌胃,实验组予活血荣络片药液灌胃,对照组以磷酸吡哆醛丁咯地尔胶囊药液灌胃,均造模6 h后首次给药,然后每日灌胃2次,直至处死为止。每日观察大鼠的一般情况,进行神经功能缺损评分,并分别在造模1、3、5及7 d后各组随机选取6只大鼠处死取材,观察比较各组大鼠脑组织Caveolin-1的表达情况。结果造模1 d后,造模组各组大鼠神经功能缺损评分比较差异无统计学意义(P0.05);造模3、5及7 d后对照组及实验组大鼠神经功能缺损评分与模型组比较差异有统计学意义(P0.05),均高于模型组,且对照组与实验组组间比较差异均无统计学意义(P0.05);模型组、对照组及实验组造模1、3、5及7 d后Caveolin-1表达水平与假手术组同期比较差异均有统计学意义(P0.05),均高于假手术组;对照组及实验组造模1、3、5及7 d后Caveolin-1表达水平与模型组同期比较差异均有统计学意义(P0.05),均高于模型组;实验组在造模1、3及5 d后时Caveolin-1表达水平与对照组比较差异无统计学意义(P0.05),在造模7 d后时Caveolin-1表达水平明显高于对照组,比较差异有统计学意义(P0.05)。结论活血荣络片可明显增加Caveolin-1在梗死周围脑组织中的表达,促进脑血管新生,从而改善脑缺血后神经功能缺损。

Effects of Huoxue - rongluo tablets on the expression of Caveolin - 1 in brain tissue of middle cerebral artety occlusion rats

Objective To study the effect of Huoxue - rongluo tablets on the expression of Caveolin - 1 in brain tissue of middle cerebral artery occlusion(MCAO)rats,and investigate nerve function recovery mechanism. Methods 96 SD rats were divided into sham operation group(24 cases)and modeling group(72 cases). Rats in modeling group were constructed in MCAO model by suture - occluded method. After successful modeling,rats in modeling group were randomly divided into model group,control group and experimental group,24 rats in each group. Rats in sham operation and model group were received distilled water(2 mL)via gastrogavage. Rats in exper-imental group were received Huoxue - rongluo liquid,and rats in control group were received buflomedil pyridoxal phosphate liquid at 6 h after modeling,twice a day until death. The daily general condition and neurological function defect score was observed. 6 rats in each group were ran-domly selected,sacrificed at 1,3,5,7 d after molding re-spectively. The expressions of Caveolin - 1 in mice brain tis-sue were observed in all groups. Results One day after modeling,there was no statistical difference on neurological function defect score in each group of modeling group(P ﹥ 0. 05). The neurological function defect scores 3,5,7 d after modeling in control and experimental group were high-er than those in model group(P ﹤ 0. 05),and there was no statistical difference between control and experimental group(P ﹥ 0. 05). The expression of Caveolin - 1 1,3,5,7 d after modeling in model control and experimental group was higher than that in sham operation group at same period(P ﹤ 0. 05). There were no statistical differences on Caveolin - 1 expression in experimental and control group at 1,3 and 5 d after modeling(P ﹥ 0. 05). The expres-sion of Caveolin - 1 in experimental group 7 d after modeling was obviously higher than that in control group(P ﹤0. 05). Conclusion Huoxue - rongluo tablets can significantly increased the expression of Caveolin - 1 in brain tis-sue around infarction,can promote cerebral vascular growth then improve the neurological function.