神经生长因子和重组骨形态蛋白对人牙周膜细胞增殖与分化作用的影响

目的探讨神经生长因子(NGF)和重组骨形态蛋白(rhBMP)分别及联合作用对体外培养的人牙周膜细胞(hPDLCs)增殖和碱性磷酸酶(ALP)活性的影响。方法采用小鼠骨髓微核试验。体外培养hPDLCs,与不同浓度的NGF、rhBMP或NGF+rhBMP作用,用噻唑盐(MTT)法观察PDLC增殖的情况,用APL活性检测法检测ALP的活性。结果NGF、rhBMP都可促进人PDLC的增殖及ALP的活性,这种促进作用呈一定的浓度依赖性。NGF与rhBMP联合应用对人PDLC的增殖及ALP的活性有协同作用,与单独应用相比差异有统计学意义。结论NGF、BMP都可促进人PDLC的增殖及ALP的活性,且二者联合具有协同作用。

Effect of nerve growth factor and recombinant human bone morphogenetic protein on the proliferation and alkaline phosphatase activity of human periodontal ligament cells

Objective To evaluate the in vitro biological effects of nerve growth factor and recombinant human bone morphogenetic protein on the proliferation and alkaline phosphatase activity of human periodontal ligament cells.Methods Human periodontal ligament cells were cultured by tissue explant and its proliferation were measured by MTT colorimetric assay.ALPase activity was measured by enzyme kinetic methods.Data was analyzed by ANOVA.Results NGF or rhBMP-2 can stimulate human PDL cells proliferation in dose-dependent manner.The co-ordinate use of NGF and rhBMP-2 has synergetic effect on PDL cells proliferation.Conclusion NGF and rhBMP-2 can be used as bioactive mediators on periodontal regeneration.