卵巢癌及癌旁组织端粒酶活性与凋亡相关性研究

目的研究端粒酶活性和抗凋亡基因Bcl-2及促凋亡基因Bax在卵巢癌发生、发展过程中的变化。方法应用SP免疫组织化学方法,对50例卵巢癌及癌旁正常组织进行hTERT、Bcl-2、Bax基因蛋白的检测。结果hTERT和Bcl-2阳性表达率在卵巢癌组织中高于癌旁组织。而Bax阳性表达率在卵巢癌组织低于癌旁组织,2组间均有明显差异。hTERT蛋白表达阳性率在临床Ⅰ、Ⅱ期和临床Ⅲ、Ⅳ期分别为16/23(69.56%)和26/27(96.30%),差异有显著性(P<0.05)。高分化组织与低分化组织中阳性率分别为18/23(78.26%)和25/27(92.59%),差异也有显著性(P<0.05)。对于Bcl-2蛋白表达阳性率在临床Ⅰ、Ⅱ期和Ⅲ、Ⅳ期中分别为8/23(34.78%)和16/27(59.26%),高分化组织和低分化组织阳性率分别为9/23(39.13%)和18/27(66.67%),差异均有显著性(P<0.05)。卵巢癌组织凋亡指数明显低于卵巢癌旁组织,差异有显著性(P<0.05)。结论凋亡调控功能失调与肿瘤的形成有关,hTERT、Bcl-2、Bax基因蛋白的异常表达与卵巢癌发生有关;hTERT、Bcl-2在卵巢癌的发生发展中可能有协同作用,hTERT、Bcl-2与Bax在卵巢癌的发生发展中可能有拮抗作用。hTERT、Bcl-2蛋白的表达可能是反映卵巢癌生物学行为的重要参数。

A study on correlation between ovarian cancer telomerase activity and apoptosis

Objective To study the change of telomerase activation and anti-apoptosis gene Bcl-2 and apoptosis gene Bax during the process of ovarian cancer.Methods immunohistochemistry was used as the method.hTERT,Bcl-2,and Bax of 50 cases of ovaries and adjacent tissues were examined.Results The positive rate of hTER and Bcl-2 was higher than those of adjacent tisse.While positive rate of Bax in ovarian cancer was lower than that in the adjacent tissues.the difference was significant.the expression rate of hTERT 69.56% in clinical phase Ⅰ,Ⅱ and 96.30% in clinical phase of Ⅲ,Ⅳ demonstrated the difference was significant.The rates of hTERT in well-differentiated and poorly differentiated tissues were 78.26% and 92.59%.The difference was significant.The expression rate of Bcl-2 in clinical phase Ⅰ,Ⅱ and clinical phase Ⅲ,Ⅳ was 34.78% and 59.26% respectively.the positive rate of well-differentiated and poorly differentiated tissues were 39.13% and 66.67% respectively.The difference was significant.Apoptotic index of ovarian cancer was lower than that of the adjacent tissues.Conclusions Functional disorder of apoptosis was related to the tumor.The abnormal expressions of hTERT,Bcl-2 and Bax were related to the genesis of ovarian cancer.While hTERT and Bcl-2 was conducive to the tumor.hTERT,Bcl-2 and Bax can prevent the genesis of ovarian cancer.The expressions of hTERT and Bcl-2 were important parameter that reflects the biological character of ovarian cancer