| 氯胺酮对脓毒症大鼠肾脏保护作用的研究 |
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| 引用本文： | 秦鑫,陈丽.氯胺酮对脓毒症大鼠肾脏保护作用的研究[J].中国医疗前沿,2011(2):32-33,29. |
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| 作者姓名： | 秦鑫 陈丽 |
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| 作者单位： | 山西医科大学第二医院麻醉科; |
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| 摘 要： | 目的探讨氯胺酮对脓毒症大鼠肾脏的保护作用及其机制。方法选健康雄性Wistar大鼠54只,随机分为三组：假手术组（SHAM）组、盲肠结扎加穿孔组（CLP）组及氯胺酮组（KT）组。SHAM组开腹后不做任何处理,其余各组行盲肠结扎穿孔术（CLP）。KT组于CLP前20min腹腔给药40mg/kg。各组在3h、6h和12h分别经心脏采血,检测尿素氮和肌酐的含量,并取肾脏行HE染色观察其病理组织学变化,用免疫组化方法检测热休克蛋白70（HSP70）的表达。结果与SHAM组比较,CLP组大鼠血清BUN含量在术后6h和12h显著升高（P〈0.01）,血清Cr在术后12h显著升高（P〈0.01）;与CLP组比较,KT组大鼠血清BUN较CLP组含量在6、12h时相点明显降低（P〈0.05）。血清Cr在12h明显降低（P〈0.05）。三组肾组织中HSP70均有表达,CLP组及KT组均明显高于SHAM组（P〈0.01）,而KT组明显高于CLP组（P〈0.05）。结论氯胺酮能抑制脓毒症大鼠血清BUN和Cr的增高,减轻肾组织损害,对脓毒症大鼠肾脏具有一定的保护,此作用可能与诱导HSP70表达增强有关。
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| 关 键 词： | 氯胺酮 热休克蛋白70 脓毒症 肾功能 |
The effects of Ketamine on renal function in septic rats |
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| QIN Xin,CHEN Li.The effects of Ketamine on renal function in septic rats[J].China Healthcare Innovation,2011(2):32-33,29. |
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| Authors: | QIN Xin CHEN Li |
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| Institution: | QIN Xin,CHEN Li.Department of Anesthesiology,The Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective It is to study the renal protective effect and its mechanism of Ketamine(KT) on rats with sepsis.Methods Rat sepsis model was established by using cecal ligation and puncture(CLP).Fifty-four Male Wistar rats were randomly divided into SHAM group,group CLP and group KT.SHAM group received laparotomy without any treatment,other groups underwent CLP group.In KT group at 20 min after CLP the intraperitoneal administration of 40mg/kg.Blood of heart was sampled for detection of renal function at 3h,6h a... |
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| Keywords: | Ketamine HSP70 Sepsis Renal function |
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