舒芬太尼后处理对大鼠肠缺血再灌注时肝组织损伤的影响

目的研究舒芬太尼后处理对大鼠肠缺血再灌注时肝组织损伤的影响。方法成年Wista大鼠40只,随机分为5组（n=8）：假手术组（S组）、肠缺血再灌注组（IIR组）、舒芬太尼后处理低、中、高剂量组（SP1、SP2、SP3组）。于再灌注3h时处死大鼠,取肝组织,用免疫组织化学方法检测Bcl-2与Bax表达水平,用TUNEL法检测并计算肝细胞凋亡指数（AI）。结果与S组相比,IIR组和SP1-3组Bcl-2与Bax表达上调,肝细胞AI升高,IIR组Bcl-2/Bax比值降低,SP1-3组Bcl-2/Bax比值升高（P〈0.05）;与IIR组相比,SP1-3组Bcl-2表达、Bcl-2/Bax比值升高,Bax表达下调,肝细胞AI降低（P〈0.05）。结论舒芬太尼后处理可抑制大鼠肠缺血再灌注时肝组织损伤,其机制可能与上调Bcl-2表达及抑制Bax上调表达有关。

Effects of Sufentanil post-conditioning on hepatocellular apoptosis and expression of Bcl-2,Bax induced by intestinal ischemia-reperfusion in rats

Objective To investigate the effects of sufentanil postconditioning liver tissue injury induced by intestinal ischemiareperfusion in rats.Methods Forty Wistar rats were randomly divided into 5 groups（n=8）：Shamoperation group（S group）,intestinal ischemia reperfusion group（IIR group） and postconditioning of low-dosage sufentanil group（SP1 group）、middle-dosage group（SP2 group） and high-dosage group（SP3 group）.After reperfusion two hours,Immunohistochemistry was used to detect the Bcl-2 and Bax protein of hepatic tissue.The presence of apoptosis was detected by TUNEL.Results Compared with the sham-operated group,express-ion of Bax,Bcl-2 and apoptosis index of hepatic cell increased in intestinal ischemia reperfusion group and sufentanil postconditioning group.Bcl-2/Bax ratio is lower in intestinal ischemi-a-reperfusion group.Bcl-2/Bax ratio is higher in sufentanil postconditioning group（P0.05）.Compared with the intestinal ischemia reperfusion group,expression of Bcl-2 and Bcl-2/Bax ratio is higher and expression of Bax and apoptosis index of hepatic cell is lower in sufentanil postconditioning group（P0.05）.Conclusion Sufentanil postconditioning can protect liver injury induced by intestinal ischemia-reperfusion in rats.One of mechanisms of protection is may be that Sufentanil increase the expression of Bcl-2 protein and decrease the expression of Bax protein.