| HPLC-MS法测定白花丹参中丹酚酸B的含量 |
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| 引用本文： | 李福荣,史卫锋,夏作理.HPLC-MS法测定白花丹参中丹酚酸B的含量[J].中国药房,2007,18(21):1637-1638. |
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| 作者姓名： | 李福荣 史卫锋 夏作理 |
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| 作者单位： | 1. 泰山医学院药学院,泰安市,271016
2. 泰山医学院生命科学研究所,泰安市,271000 |
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| 基金项目： | 山东省中医药管理局资助项目 |
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| 摘 要： | 目的:建立以高效液相色谱-质谱法测定白花丹参中丹酚酸B含量的方法。方法:色谱柱为Symmetry field RP C_(18) (150mm×2.1mm,3.5μm),流动相为甲醇水,流速为0.2mL·min~(-1),检测波长为285nm;质谱为Waters micromass ZQ4000质谱仪,离子模式为选择离子(SIR)。结果:丹酚酸B在0.05～60μg·mL~(-1)浓度范围内与峰面积积分值呈良好线性关系(r=0.999 8);平均加样回收率为99.18%,RSD=0.44%(n=3)。结论:本方法可为白花丹参的质量评价和道地药材质量标准的建立提供理论依据。
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| 关 键 词： | 高效液相色谱-质谱 白花丹参 丹酚酸B 含量测定 |
| 文章编号： | 1001-0408（2007）21-1637-02 |
| 收稿时间： | 2007-01-11 |
| 修稿时间： | 2007-01-112007-03-12 |
Determination of Salvianolic Acid B in Salvia miltiorrhiza Bge.f.alba by HPLC-MS |
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| LI Furong,SHI Weifeng,XIA Zuoli.Determination of Salvianolic Acid B in Salvia miltiorrhiza Bge.f.alba by HPLC-MS[J].China Pharmacy,2007,18(21):1637-1638. |
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| Authors: | LI Furong SHI Weifeng XIA Zuoli |
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| Abstract: | OBJECTIVE:To determine Salvianolic acid B in Salvia miltiorrhiza Bge.f.alba by HPLC-MS.METHODS: The sample was separated on Symmetry field RP C_(18)(150mm×2.1mm,3.Sμm),column.The mobile phase consisted of methanol-water with a flow rate of 0.2mL·min~(-1).The detection wavelength was 285nm;the MS was Waters micromass ZQ4000,and the ion mode was SIR ion.RESULTS:The linear range of Salvianolic acid B was 0.05～60μg·mL~(-1)(r=0.9998), and the average recovery was 99.18%,RSD=0.44%(n=3).CONCLUSION:The determination method has provided a theoretical basis for the quality evaluation of Salvia miltiorrhiza Bge.f.alba and establishment of quality criteria of genuine drugs. |
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| Keywords: | HPLC-MS Salvia miltiorrhiza Bge f alba Salvianolic acid B Content determination |
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