三磷酸腺苷诱导体外培养的胚胎大鼠皮层神经细胞凋亡

目的：研究不同浓度三磷酸腺苷（ATP）溶液（0.1、10、100?mmol/L）对体外培养的胚胎大鼠皮层神经细胞的作用。方法：应用倒置显微镜和活细胞计数观察神经细胞死亡性质和细胞存活量；采用Neuron Specific Enolase (NSE) 和cleaved caspase 3免疫组织化学方法了解各实验组NSE阳性神经元的数量和凋亡特异蛋白caspase 3活化情况。结果：10?mmol/L及100?mmol/L ATP溶液作用细胞4?h，存活细胞数与NSE阳性神经元数量呈浓度依赖性减少，与对照组相比,差异有统计学意义（P＜0.01）。10?mmol/L ATP作用细胞4?h,活化的 caspase 3蛋白表达较对照组显著性增加（P＜0.05）。结论:ATP对体外培养的胚胎大鼠皮层神经细胞具有浓度依赖性的细胞毒性作用，细胞凋亡蛋白caspase 3活化机制参与该过程。

Adenosine triphosphate promotes the apoptosis in cultured cerebral cortex neurons of embryonic rats

To explore the role of adenosine triphosphate (ATP) of different concentrations in cultured cerebral cortex neurons of embryonic rats. Methods: The inverted microscope was used to observe the nature of dead cells and to count the number of the cells. Neuron specific enolase(NSE) and cleaved caspase 3 immunohistochemistry technique was used to determine the number of NSE positive neurons and to observe the expression of caspase 3 protein. Results: Both the number of cultured cells and the NSE positive neurons decreased obviously with ATP (10?mmol/L and 100?mmol/L) treatment, but the number of the cleaved caspase 3 positive neurons increased obviously. Conclusion: ATP has dose dependent cyto toxicity on cultured cerebral cortex neurons of embryonic rats and caspase 3 signal is necessary for the cell death.