三氧化二砷亚急性染毒对雄性小鼠生殖与免疫毒性的研究

目的探讨As2O3亚急性染毒对雄性小鼠生殖与免疫毒性作用。方法24只清洁级KM雄性小鼠,分为对照组、低、中及高剂量染砷组。分别给予去离子水,As2.0、4.0、8.0mg/kg体重灌胃染毒7天。实验结束后剖杀动物取睾丸、附睾称重、计算器官指数并测定附睾精子数,摘取免疫器官胸腺、脾脏称重、计算免疫器官指数。结果(1)高剂量组睾丸及附睾重量低于正常对照组(P<0.05),但各组睾丸指数与对照组没有差异;各染砷组精子数均低于对照组(P>0.05),且中、高剂量组精子畸形率上升显著(P<0.05);(2)中、高剂量组免疫器官重量及指数降低,且高剂量组脾脏重量及指数显著低于对照组(P<0.05)。结论As2O3可致小鼠某些生殖和免疫毒性指标改变。

Study on reproductive and immune toxicity of male rats exposed to As2O3

Objective To study on reproductive and immune toxicity of male rats exposed to subacute As_2 O_3. Methods 24 male rats of KM-strain were divided into four groups and were given by gavage deionized water,2.0,4.0 and 8.0 mg As/(kg·bw) for 7 days. After the exposure, the rats were killed and the testis, epididymis were collected and analysed. Results (1) In comparition with control, testicular and epididymal weights were lower than those of high dose group ( P < 0.05), but there were no difference of testicular indexes between exposed group and control. The stained sperm count were lower than those of arsenic exposure group ( P > 0.05), and malformation rate of sperm were significant increase in middle-and high-dose group ( P < 0.05); (2)Compared to control, the weight and index of immune organ was decrease in middle-and high-dose group, and the spleen weight and index was significantly lower in high-dose group (P < 0.05) . Conclusion As_2 O_3 can induce reproductive and immunotoxicity in male mice.