| 随机引物扩增技术对变形菌DNA多态性分型研究 |
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| 引用本文： | 马清光,赵月辉.随机引物扩增技术对变形菌DNA多态性分型研究[J].中华医院感染学杂志,2005,15(1):26-28. |
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| 作者姓名： | 马清光 赵月辉 |
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| 作者单位： | 1. 上海市大场医院,上海,200436
2. 哈尔滨医科大学微生物教研室,黑龙江,哈尔滨,150060 |
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| 摘 要： | 目的建立变形菌随机扩增DNA多态性分型技术,应用于临床菌株流行病学调查. 方法优化随机扩增DNA多态性指纹技术(RAPD)的实验条件,利用RAPD技术成功地检测21株变形菌DNA指纹图谱. 结果 21株变形菌分为18型别,其中16株奇异变形菌分为13个型,5株普通变形菌分为5个型. 结论 RAPD分型技术可简便、快速为变形菌提供分型标志,是分子流行病学研究的有效方法.
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| 关 键 词： | 随机引物PCR 变形菌属 DNA多态性 |
| 文章编号： | 1005-4529(2005)01-0026-03 |
| 修稿时间： | 2004年1月15日 |
Typing of Proteus Strains by Random Amplification of Polymorphic DNA |
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| MA Qing-guang,ZHAO Yue-hui.Typing of Proteus Strains by Random Amplification of Polymorphic DNA[J].Chinese Journal of Nosocomiology,2005,15(1):26-28. |
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| Authors: | MA Qing-guang ZHAO Yue-hui |
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| Abstract: | OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation. METHODS To optimize the experiment condition of random amplification DNA polymorphic fingerprinting technique. Fingerprinting map of 21 Proteus strains were tested by RAPD. RESULTS Twenty one Proteus strains were divided into 18 distinct genotypes, among them 16 P. mirabilis strains were divided into 13 distinct genotypes and 5 from P. vulgaris strains. CONCLUSIONS RAPD provides markers for typing of clinical strains and it is suitable for molecular epidemiology studies with high typeability, simplicity and rapidness. |
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| Keywords: | Arbitrarily primed polymerase chain reaction Proteus RAPD |
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