| 宋内氏福氏2a双价志贺氏菌芳香族氨基酸营养缺陷型减毒株的构建 |
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| 引用本文： | 刘士辉,毛培基,牟兆钦,王敖全. 宋内氏福氏2a双价志贺氏菌芳香族氨基酸营养缺陷型减毒株的构建[J]. 中华微生物学和免疫学杂志, 1994, 0(3) |
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| 作者姓名： | 刘士辉 毛培基 牟兆钦 王敖全 |
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| 作者单位： | 北京军事医学科学院微生物流行病研究所，中国科学院微生物研究所，北京军事医学科学院生物工程研究所 |
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| 摘 要： | ＦＳ５４是本室构建的宋内氏福氏２ａ双价志贺氏菌杂交株，具有志贺氏菌的毒力。本研究通过遗传重组，给ＦＳ５４株引入了ａｒｏＤ基因的转座于Ｔｎ１０插入失活物（ａｒｏＤ：：Ｔｎ１０），构建了ＦＳ５４株的芳香族氨基酸营养缺陷型减毒株，并进一步去除了Ｔｎ１０所携带的四环素抗性和宋内氏１相大质粒（ｐＳＳ１２０：：Ｔｎ５）上所携带的卡那霉素抗性，得到了宋内氏福氏２ａ双价志贺氏菌芳香族氨基酸营养缺陷型减毒株Ｆｓ５４－７ｂ．
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| 关 键 词： | 志贺氏菌；芳香族氨基酸营养缺陷型；减毒活菌株 |
CONSTRUCTION OF AN AROMATIC ACID AUXOTROPHIC ATTENUATED STRAIN OF THE BIVALENT HYBRID STRAIN(FS54)OF S. SONNEI AND S. FLEXNERI 2A |
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| Liu Shi-hui,Mao Pe-ji,Mu zhao-qin,et al. CONSTRUCTION OF AN AROMATIC ACID AUXOTROPHIC ATTENUATED STRAIN OF THE BIVALENT HYBRID STRAIN(FS54)OF S. SONNEI AND S. FLEXNERI 2A[J]. Chinese Journal of Microbiology and Immunology, 1994, 0(3) |
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| Authors: | Liu Shi-hui Mao Pe-ji Mu zhao-qin et al |
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| Abstract: | he virulent strain of FS54 constructed in our laboratory is a bivalent hybrid strain of S.sonnei and S.flexneri 2a. In this paper,we made a series of aromatic acid auxotrophic attenuated strains by introducing Tn 10 inactivatedaroD gene into FS54 strain. Furthermore ,we successfully eliminated the tetracycline resistant gene (carried byTn 10)and kanamycin resistant gene(carried by Tn5 on form I plasmid pSS120::Tn5),and eventually obtained aTc sensitive,Km sensitive aromatic-dependent attenuated bivalent strain of S. sonnei and S. flexneri 2a,i.e. FS54-7b strain. |
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| Keywords: | Shigella Aromatic acid auxotrophy Attenuated strain |
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