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Increased expression of integrin α2 and abnormal response to TGF-β1 in hereditary gingival fibromatosis |
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| Authors: | J Zhou LY Meng XQ Ye JW Von den Hoff Z Bian |
| Institution: | Key Laboratory for Oral Biomedical Engineering Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China;;Department of Orthodontics and Oral Biology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands |
| Abstract: | Objective: To investigate the possible correlation between integrin α1, α2, and β1 expression and excessive collagen synthesis in fibroblasts from 3 unrelated Chinese families with hereditary gingival fibromatosis (HGF). Design: Gingival fibroblasts from three Chinese HGF patients and three healthy subjects were included. The expression of α1, α2, and β1 integrin subunits was examined by immunohistochemistry, quantitative PCR, and flow cytometry. We also investigated the effects of transforming growth factor-β1 (TGF-β1) on the expression of these integrin subunits. Results: Our results demonstrate that the expression of α2 was significantly higher in HGF fibroblasts compared with control fibroblasts ( P < 0.01). No significant differences in the expression of α1 and β1 were detected. Furthermore, TGF-β1 promoted the expression of α1 and α2 in fibroblasts from both HGF patients and controls. However, it had a larger effect on the expression of α2 in HGF fibroblasts than in control cells. In contrast, α1 expression was stimulated more in control fibroblasts. Conclusion: The increased expression of integrin α2 and the increased response to TGF-β1 of HGF fibroblasts may be related to the excessive collagen deposition in HGF patients. |
| Keywords: | hereditary gingival fibromatosis integrin expression TGF-β1 |