GFAPδ immunostaining improves visualization of normal and pathologic astrocytic heterogeneity |
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| Authors: | Felipe Andreiuolo Marie‐Pierre Junier Elly M Hol Catherine Miquel Leila Chimelli Nadine Leonard Hervé Chneiweiss Catherine Daumas‐Duport Pascale Varlet |
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| Institution: | 1. Department of Pathology, Centre Hospitalier Sainte‐Anne, Université Paris Descartes,;2. Department of Pathology, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;3. INSERM U834;4. Université Paris Descartes;5. Centre Hospitalier Sainte‐Anne, Paris, France;6. Netherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands;7. and |
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| Abstract: | Neuropathological analysis of cellular mechanisms underlying gliosis and brain tumors is slowed by the lack of markers allowing to distinguish glial subpopulations in normal or pathological human brains. We therefore evaluated GFAPδ immunostaining in a wide panel of astrogliosis and gliomas, and compared these with GFAP and vimentin. In normal tissue, gliosis and gliomas, GFAPδ immunostaining was observed in astrocytes with relatively high GFAP levels. GFAPδ immunostaining was most conspicuous in glia limitans astrocytes. In Chaslin's gliosis accompanying chronic epilepsy, GFAPδ immunostaining evidenced the glia limitans reactive astrocytes as the source of the dense fibrillary meshwork typical of Chaslin's gliosis. Interestingly GFAPδ and vimentin immunostainings coincided in normal tissues and gliosis, but not in gliomas. Altogether these results show that combined GFAP, GFAPδ and vimentin labelling reveals fine gliofilament regulation in normal and pathological brain. |
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| Keywords: | GFAP delta glia limitans gliomas gliosis intermediate filaments |
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