| 腺病毒DNA对肝细胞系的转化活性的研究 |
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| 引用本文： | 孟运莲,Craig Woodworth,Harriet C Isom.腺病毒DNA对肝细胞系的转化活性的研究[J].武汉大学学报(医学版),1992(1). |
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| 作者姓名： | 孟运莲 Craig Woodworth Harriet C Isom |
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| 作者单位： | 湖北医学院组胚教研室，美国宾州州立大学医学院，美国宾州州立大学医学院 |
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| 摘 要： | 用含有腺病毒EIA和EIB基因的DNA片段对一株由猴病毒40(SV_(40))转化的大鼠肝细胞系——CWSV-1细胞进行了转化实验,以了解腺病毒基因和SV_(40)DNA之间的关系。我们的结论是:(1)腺病毒DNA能转化CWSV-1细胞;(2)建立的NP细胞呈重叠生长趋势,当细胞复盖培养基达60％左右时,大部份细胞自行脱落,但细胞形态没有明显改变;(3)形成的NP细胞系大部份不再分泌可检测的白蛋白,少量细胞分泌白蛋白的能力也随着传代而消失。
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| 关 键 词： | 基因 病毒 转染 肝/解剖学和组织学 |
The Conversion Activation of Adenovirus DNA on a Hepatocyte Cell Line |
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| Meng Yunlian,Craig Woodworth,Harriet C Isom.The Conversion Activation of Adenovirus DNA on a Hepatocyte Cell Line[J].Medical Journal of Wuhan University,1992(1). |
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| Authors: | Meng Yunlian Craig Woodworth Harriet C Isom |
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| Institution: | Meng Yunlian,Craig Woodworth,Harriet C Isom Department of Histology and Embryology,Hubei Medical College,Wuhan,China 430071 Department of Microbiology and Immunology,Pannsylvania State College of Medivine,USA |
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| Abstract: | The plasmid which carried adenovirus DNA was used to transform a low passage SV_(40)-immortal- ized hepatocyte cell line(CWSV-1)to find out the relations between the virus genes.We conclude. that:(1)CWSV-1 cells can be transformed by transfection with adenovirus DNA;(2)The trans- formed cells by adenovirus DNA(NP cells)grow in a overlapping way.The cells come off when they cover 60％ of dish;(3)The NP cells do not retain the ability to express detectable albumin. |
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| Keywords: | MeSH genes viral transfection liver/AH |
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