| 三种鼠脑胶质瘤模型的建立与比较 |
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| 引用本文： | 朱侗明,何升学,胡新华,章文斌.三种鼠脑胶质瘤模型的建立与比较[J].立体定向和功能性神经外科杂志,2011(3):144-148. |
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| 作者姓名： | 朱侗明 何升学 胡新华 章文斌 |
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| 作者单位： | 南京医科大学附属脑科医院神经外科;江苏大学附属镇江第一人民医院神经外科; |
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| 基金项目： | 南京市科技计划发展项目(编号:200902018); 江苏省卫生厅课题基金项目(编号:Z201010) |
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| 摘 要： | 目的 ;建立GL261胶质瘤细胞C57BL/6小鼠、C6胶质瘤细胞SD大鼠及BALB/c小鼠皮下动物模型,比较其肿瘤生长特点。方法借助动物立体定向仪,将体外培养小鼠GL261、大鼠C6胶质瘤细胞分别接种于C57BL/6小鼠及SD大鼠右侧尾状核区,C6胶质瘤细胞接种于BALB/c小鼠左前肢皮下。接种后观察不同种实验鼠的生存状态及肿瘤的生长特性,颅内模型于接种后7d、14d、21d、28d进行MRI检查,皮下模型测量体积,并绘制生长曲线。解剖标本,做组织病理学和胶质纤维酸性蛋(GFAP)免疫组化检查。结果 GL261胶质瘤细胞C57BL/6小鼠模型较之后两种模型在组织病理学上接近人脑胶质瘤,而且颅内生长稳定,成瘤率高,未见颅外转移病灶,实验周期短,重复性好。结论 GL261胶质瘤细胞C57BL/6小鼠模型,其肿瘤生长特性及病理特征与人脑胶质瘤相似,可作为临床胶质瘤基础研究的理想模型。
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| 关 键 词： | 胶质瘤 立体定向技术 动物模型 |
Establishment and comparison of three brain-glioma models of murine |
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| Zhu Tongming,He Shengxue,Hu Xinhua,et al..Establishment and comparison of three brain-glioma models of murine[J].Chinese Journal of Stereotactic and Functional Neurosurgery,2011(3):144-148. |
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| Authors: | Zhu Tongming He Shengxue Hu Xinhua |
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| Institution: | Zhu Tongming,He Shengxue,Hu Xinhua,et al.Department of neurosurgery,Brain Hospital affiliated to Nanjing Medical University,Nanjing,210029,China |
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| Abstract: | Objective To explore tumor growth characteristics of mouse glioma GL261 model and SD,BALB/c C6 glioma models.Methods The glioma cells cultured in vitro were stereotaxically implanted into right caudate nucleus of C57BL/6 mouse and SD rats,C6 cells were implanted into subcutaneous of BALB/c mouse.The living and survival states were Observed.After inoculation,use an MRI protocol to evaluate intracranial tumor growth on 7,14,21,28 day.Measure tumor volume of subcutaneous model,and draw the growth curve.Animals... |
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| Keywords: | Glioma Stereotatic technique Animal model |
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