| 小鼠脑皮质撞击模型的构建和评估 |
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| 引用本文： | 张明坤,钟春龙,崔振文,吴增宝,徐巳奕,郑彦,王勇,江基尧,罗其中.小鼠脑皮质撞击模型的构建和评估[J].立体定向和功能性神经外科杂志,2011(3):153-156. |
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| 作者姓名： | 张明坤 钟春龙 崔振文 吴增宝 徐巳奕 郑彦 王勇 江基尧 罗其中 |
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| 作者单位： | 上海交通大学医学院附属仁济医院神经外科; |
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| 基金项目： | 国家自然科学基金项目(编号:30500525,30670719,81070990); 上海市科委基金项目(编号:09140901300,10QH1401700); 上海市教委基金项目(编号:09SG20) |
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| 摘 要： | 目的 ;探索建立小鼠脑皮质撞击模型,为今后进一步开展小鼠颅脑创伤研究创造条件。方法雄性C57BL/6J小鼠36只,随机分为6组(包括实验组5组和对照组1组),应用NYU Impactor Ⅰ型脑皮质撞击仪,金属杆(重10g)从不同高度落下垂直打击小鼠脑皮质造成损伤,建立小鼠脑皮质撞击模型。致伤后观察小鼠神经反射及意识恢复时间。伤后24 h处死小鼠,取海马CA2/3区脑组织行退变神经元Fluoro-Jade B组织荧光染色,通过立体细胞计数观察退变神经元数量变化。结果打击高度为10~40 mm时,损伤侧脑组织病理改变的严重程度逐级加重,与损伤程度存在较好的相关性;打击高度为10 mm时可近似模拟轻型颅脑损伤;打击高度为20~30 mm时近似模拟中型颅脑损伤;打击高度为40 mm时近似模拟重型颅脑损伤。结论应用NYU Impactor Ⅰ型脑皮质撞击仪可以成功地制作出所需的小鼠脑皮质撞击模型,具有重复性好、简便易用的特点,是较理想的小鼠致伤模型。
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| 关 键 词： | 创伤性脑损伤 脑皮质撞击 动物模型 小鼠 |
The establishment and evaluation of the cortical impact injury model in the mouse |
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| Zhang Mingkun,Zhong Chunlong,Cui Zhenwen,et al..The establishment and evaluation of the cortical impact injury model in the mouse[J].Chinese Journal of Stereotactic and Functional Neurosurgery,2011(3):153-156. |
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| Authors: | Zhang Mingkun Zhong Chunlong Cui Zhenwen |
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| Institution: | Zhang Mingkun,Zhong Chunlong,Cui Zhenwen,et al.Department of Neurosurgery,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai,200127,China |
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| Abstract: | Objective To establish the cortical impact injury model in the mouse by using the type 1 NYU Impactor.We believe that the works would help to build on our experience in the future traumatic brain injury researches in the mouse.Methods A total of 36 male C57BL/6J mouse were divided into 6 groups and were named control(1 group)and TBI groups(5 groups)respectively.There were 6 animals in each group.The falling metal rod(10 grams) produced various injury levels by directly impacting the mouse cortex from the di... |
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| Keywords: | Traumatic brain injury Cortical impact Animal model Mouse |
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