Different responses of three rodent Plasmodia species,Plasmodium yoelii 17XL,P. berghei NK65 and P. chabaudi AS on treatment with febrifugine and isofebrifugine mixture from Hydrangea macrophylla var. Otaksa leaf in ICR mice

The antimalarial activity of Hydrangea macrophylla var. Otaksa alkaloids was evaluated against Plasmodium yoelii 17XL, P. berghei NK65 and P. chabaudi AS in ICR mice. For trials in P. yoelii 17XL or P. chabaudi AS infections, mice were infected intraperitoneally with 10(5), 10(6) and 10(7) parasitized erythrocytes, respectively, and in P. berghei NK65 infections, mice were infected intraperitoneally with 10(3), 10(4) and 10(5) parasitized erythrocytes, respectively. Three days after injection, mice were orally given febrifugine and isofebrifugine mixture at 1 mg/kg in the treated group and 0.5% cremophor EL solution in the untreated, infected one, respectively, twice a day for 5 consecutive days. In P. yoelii 17XL infections, mice in all the non-treated controls died from 5 to 9 dpi with a gradual body weight loss and increasing parasitemias. In the treated groups, the mouse body weight gradually decreased after the end of administration but turned to increase in several days, and except one mouse in the group given 10(6) parasitized erythrocytes, other mice survived during the experiment. Mice given orally the mixture showed low parasitemia levels during administration. Following a transient recrudescence of malaria parasites in the bloodstream of treated mice, no parasites could be detected by a microscopic examination. In P. berghei NK65 infections, mice in all the non-treated controls died from 7 to 12 dpi with a gradual body weight loss and increasing parasitemias. In the treated groups, the body weight gradually decreased from 11 dpi and all mice died from 12 to 30 dpi. During a mixture administration all mice showed slight suppression of multiplication of malaria parasites. After the end of administration, however, malaria parasites increased in the bloodstream of the treated mice and all mice died. In P. chabaudi AS infections, there were two different patterns in the course of infection; lethal infection or recovery in both the non-treated control and treated groups. In the non-treated and treated groups, mice showed a gradual body weight loss. But the body weights of survivals in both groups turned to increase in several days. Mice in control and treated groups showed as the same profile in the changes of parasitemia. In the non-treated controls, after a transient peak parasitemia malaria parasites in the bloodstream of survivals could not be detected by a microscopic examination. During a mixture administration, all mice showed suppression of multiplication of malaria parasites. After the end of medication, some mice died with increasing parasitemia. After a transient recrudescence, however, malaria parasites in the bloodstream of survivals could not be detected by a microscopic examination.