高效液相色谱法同时测定黄芩及其制剂中4种黄酮的含量

目的建立反相高效液相色谱法同时测定黄芩药材及其制剂中黄芩苷（1）、汉黄芩苷（2）、黄芩素（3）和汉黄芩素（4）的含量。方法采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）;以乙腈-0.15%三氟乙酸水为流动相,梯度洗脱;流速：1.0 ml·min^-1;检测波长：280 nm;柱温：室温。结果已测定的4种黄酮在以下浓度范围内呈现良好的线性：（1）0.51～164 mg·L^-1（r=0.9999）,（2）0.33～104 mg·L^-1（r=0.9998）,（3）0.24～76 mg·L^-1（r=0.999 8）,（4）0.31～100mg·L^-1（r=0.9999）。平均回收率（n=3）分别为：（1）98.47%（RSD=1.35%）,（2）100.73%（RSD=0.57%）,（3）101%（RSD=2.63%）,（4）100.77%（RSD=0.27%）。结论该方法简便,准确,灵敏,结果可靠,可用于黄芩药材及其制剂的质量控制。

Simultaneous determination of four flavonoids in Radix Scutellariae and its related traditional Chinese medicinal preparations by HPLC

Aim To establish a RP-HPLC method for simultaneous determination of baicalin（1）,wogonoside（2）,baicalein（3）and wogonin（4）in Radix Scutellariae and its related traditional Chinese medicinal preparations.Methods These four flavonoids were successfully analyzed on an Agilent Zorbax SB-C18 reversed-phase chromatographic column（4.6 mm × 250 mm,5 μm） by gradient elution using 0.15%（v/v） aqueous trifluoroacetic acid and acetonitrile as the mobile phase at room temperature.The flow rate was 1 ml·min^-1,and detection wavelength was set at 280 nm.Results The method showed good linearity in the ranges of（1） 0.51～164 mg·L^-1（r=0.999 9）,（2） 0.33～104 mg·L^-1（r=0.999 8）,（3） 0.24～ 76 mg·L^-1（r=0.999 8）,（4） 0.31～100 mg·L^-1（r=0.999 9） for the four flavonoids respectively;and the mean recovery（n=3） of the four invested compounds were（1） 98.47%（RSD =1.35%）,（2）100.73%（RSD=0.57%）,（3） 101%（RSD=2.63%）,（4）100.77%（RSD=0.27%）.Conclusion The developed new method is simple,precise,accurate,and reliable for quality evaluation of Radix Scutellariae and its related traditional Chinese medicinal preparations.