| 骨形成蛋白-2对人牙乳头细胞内Smad1蛋白表达量的影响 |
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| 引用本文： | 何文喜,牛忠英,赵守亮,陈健. 骨形成蛋白-2对人牙乳头细胞内Smad1蛋白表达量的影响[J]. 现代口腔医学杂志, 2002, 16(2): 132-133 |
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| 作者姓名： | 何文喜 牛忠英 赵守亮 陈健 |
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| 作者单位： | 710032,西安,第四军医大学口腔医学院牙体科 |
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| 基金项目： | 国家自然科学基金资助项目 (编号 :39870 789) |
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| 摘 要： | 目的：观察人牙乳头细胞内Smad1蛋白的表达及骨形成蛋白－2（bone morphogenetic protein-2,BMP2)对人牙乳头细胞内Smad1蛋白表达量影响，方法：原代培养的人牙乳头细胞用BMP2处理6h,12h,24h,48h后，提取总蛋白质，采用Western Bolt法，从翻译水平观察Smad1基因的表达变化。结果：从翻译水平观察到Smad1基因在人牙乳头细胞内的表达，但在BMP2作用48h内，Smad1蛋白表达量无显著变化，结论：人牙乳头细胞内存在Smad1信号转导途径，牙乳头细胞内Smad1蛋白表达量不受MBP2影响。
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| 关 键 词： | 人牙乳头细胞 信号转导 Smadl BMP2 骨形成蛋白－2 |
| 修稿时间： | 2000-09-22 |
The influence on Smad1 protein expression in the human dental papilla cells by BMP2 |
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| HE Wenxi,NIU Zhongying,ZHAO Shouliang,et al.. The influence on Smad1 protein expression in the human dental papilla cells by BMP2[J]. Journal of Modern Stomatology, 2002, 16(2): 132-133 |
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| Authors: | HE Wenxi NIU Zhongying ZHAO Shouliang et al. |
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| Affiliation: | HE Wenxi,NIU Zhongying,ZHAO Shouliang,et al. Stomatologic College,Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | Objective To investigate the expression of Smad1 gene and regulation by bone morphogenetic protein-2(BMP2)in the human dental papilla cells (HDPC).Methods Treatment by BMP2 after 6h,12h,24h and 48h,endogenous Smad1 protein expression and modulation by BMP2 in the cultured HDPC were evaluated by Western Blot analyses.Results Cultured HDPC expressed Smad1 protein on translation level.The expression of Smad1 expression wasn't significantly altered by BMP2 within 48h treatment.Conclusion Smad1 signaling is present in the HDPC,but the expression of Smad1 protein is not influenced by BMP2. |
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| Keywords: | Human dental papilla cell Signal transduction Smad1 Bone morphogenetic protein-2 |
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