血清T辅助细胞比率与妊娠期高血压疾病的关系和意义

目的探讨血清白细胞介素2（interleukin 2,IL-2）、IL-10和肿瘤坏死因子（tumor necrosis factor alpha,TNF-α）水平以及T辅助细胞1/T辅助细胞2（helper T cell1/helper T cell2,Th1/Th2）的变化与妊娠期高血压疾病发病的关系与意义。方法采用放射免疫分析法测定正常孕妇23例和52例妊娠期高血压疾病患者血清IL-2、IL-10和TNF-α的水平;分析Th1/Th2比率的变化。结果①妊娠期高血压疾病组血清IL-2和TNF-α均高于正常妊娠组,两组比较差异有显著性（P〈0.05）。妊娠期高血压疾病组内比较,差异均有显著性（P〈0.05）。血清IL-2、TNF-α的水平随疾病病情的加重而升高。②妊娠期高血压疾病组血清IL-10明显低于正常妊娠组,两组比较差异有显著性（P〈0.05）;妊娠期高血压疾病组内比较,差异无显著性（P〉0.05）。③妊娠期高血压疾病组血清中Th1/Th2比率高于正常妊娠组,两组比较差异有显著性（P〈0.05）;妊娠期高血压疾病组内比较,差异均有显著性（P〈0.05）。Th1/Th2随妊娠期高血压疾病患者病情的加重而升高。结论 Th1/Th2比率以及促进和抑制炎症的细胞因子的平衡失调,可能参与了妊娠期高血压疾病血管内皮损伤的病理生理过程,可以作为患者病情变化的指标。

The relationship and significance between serum Th1/Th2 ratio and hypertensive disorders in pregnancy

Objective To investigate the relationship between interleukin-2（IL-2）,IL-10,tumor necrosis factor alpha（TNF-α） levels,the imbalance in the helper T cell 1/helper T cell 2（Th1/Th2） ratio in patients and hypertensive disorders in pregnancy. Methods IL-2,IL-10,TNF-α levels of maternal serum from 23 healthy pregnant women（control group）,52 patients with hypertensive disorders in pregnancy（experimental group） were measured by using radioimmunoassay（RIA）; the changes of serum levels of Th1/Th2 ratio were analyzed. Results ①Serum IL-2 and TNF-α levels in experimental group were significantly higher than those in control group（P〈0.05） respectively. There was significant difference on serum IL-2 and TNF-α among experimental groups（P〈0.05）. The serum IL-2 and TNF-α levels in experimental group had the close relationship with the state of the illness. ② Serum IL-10 levels in experimental group were significantly lower than those in control group（P〈0.05）,but there was no significant difference in serum IL-10 among experimental groups（P〉0.05）. ③Th1/Th2 ratio in experimental group was significantly higher than those in control groups（P〈0.05）. There was significant difference in serum Th1/Th2 ratio among experimental groups（P〈0.05）. The Th1/Th2 ratio had a close relationship with the state of the illness,which increased in accordance with the severity of hypertensive disorders in pregnancy. Conclusion The imbalance of Th1/Th2 ratio and cytokine in patients＇ serum which were potential predictional markers of the state of hypertensive disorders in pregnancy maybe some role in pathogenic process of vascular endothelial damage of patients with hypertensive disorders in pregnancy.