兴奋性氨基酸、氧自由基与缺血再灌注脑损伤关系的实验观察

目的：观察脑缺血再灌注过程中兴奋性氨基酸（Ｅｘｃｉｔａｔｏｒｙ ａｍｉｎｏ ａｃｉｄ，ＥＡＡ）、氧自由基的变化，研究探索脑缺血再灌注损伤的机制。方法：测定假手术组、缺血３０ｍｉｎ再灌注６０ｍｉｎ生理盐水（ＮＳ）处理组和单唾液酸四已糖神经节苷脂（ＧＭ１，１０ｍｇ／ｋｇ，ＩＰ）处理组，鼠脑海马组织ＥＡＡ、丙二醛（Ｍａｌｏｎｄｉａｄｅｈｙｄｅ，ＭＤＡ）的含量。实验应用全脑缺血（４ＶＯ）模型，ＥＡＡ采用Ｈ

Experimental observation on relation of excitatory amino acid, oxygen free radicals and cerebral ischemic reperfusion damage

Objective :To ovserve the excitatory amino acid(EAA), oxygen free radicals change on cerebral ischemic reperfusion ,and study the mechanism of cerebral ischemic reperfusion damage.Methods:The hippocampus EAA,malondiadehyde (MDA) contents of sham operated group,ischemia 30 min reperfusion 60 min normal sodium (NS) and GM 1treated group GM 1,10mg/kg,IP) were measured,the global cerebral ischemic reperfusion model (4VO) was made on the experiment,EAA was measured by high performance liquid chromatogrphic detemination (HPLC),MDA was measured by TBA. Results:Compared with sham operated group,the hippocampus EAA content of cerebral ischemic reperfusion NStreated group decreases significantly (P<0.01),MDA content increases significantly (P<0.01),the hippocampus EAA and MDA contents of GM 1treated group don't change significantly (P>0.05). Conclusion:EAA,oxygen free radicals are closely related with the mechanism of cerebral ischemic reperfusion damage.