强直l生脊柱炎患者外周血T细胞凋亡及亚群极化分析

目的探讨外周血T细胞凋亡以及T细胞亚群极化在强直性脊柱炎患者免疫发病机制中的作用。方法CT检查患者骶髂关节并分为I、Ⅱ和Ⅲ级。尼龙棉枉法分离患者外周血T细胞，PI染色结合流式细胞术检测T细胞凋亡率，流式细胞术测定CD4＋CD30CF、CD4＋CD30-T、CD8＋CD30Cr、CD8＋CD30_1r细胞百分率。结果与健康对照者相比。AS患者外周血T细胞凋亡率明显降低（p〈0．05）。Ⅰ和Ⅱ级患者外周血CD4CF细胞和CD4＋T／CD8CF比值均明显降低（P〈0．05），CD8＋T细胞百分率明显升高（P〈0．05）。AS患者外周血CD4＋CD30＋T无明显变化；而CD8＋CD30CF均明显高于健康对照者（P〈0．05）。Ⅰ、Ⅱ级患者CD4＋CD30-／CD4＋CD30＋比值明显低于健康对照者（P〈0．05）；Ⅰ、Ⅱ和Ⅲ级患者CD8＊CD30-／CD8＋CD30＋比值明显低于健康对照者（P〈0．05）。结论AS患者外周血T细胞凋亡不足和T细胞亚群极化失衡与AS患者免疫学发病机制有关。

Apoptosis and polarization of T cells in ankylosing spondylitis patients

Objective To investigate the role of apoptosis and polarization of peripheral blood T cells in ankylosing spondylitis. Methods The ankylosing spondylitis patients were diagnosed in accordance with 1984 diagnostic criteria of ankylosing spondylitis and divided into grade Ⅰ , Ⅱ and Ⅲ as computerized tomography（CT）. The apoptotic ratios of T cells were evaluated by FCM. T cell subsets were analyzed by immunofluorescence staining and bicolor flow cytometry by CD4, CD8 and CD30 gating. Results The apoptotic ratios of peripheral blood T cells in AS were clearly lower than healthy control （P〈0.05）. CD4＋T cells and the ratios of CD4＋T/CD8CF in grade Ⅰ and gradeⅡ were obviously lower than healthy control （P〈0.05） while CD8＋T cells were obviously higher than healthy control （P〈0.05）. CD4＋CD30＋T cells in AS were no statistical significance with healthy control while CD8＋CD30＋T cells were obviously higher than healthy control （P〈0.05）. The ratios of CD4＋CD30-T/CD4＋CD30＋T in grade Ⅰ and Ⅱwere obviously lower than healthy control （P〈0.05）; the ratios of CD8＋ CD30＋T/CD8＋CD30＋T in all AS patients were obviously lower than healthy control （P〈0.05）. Conclusion Low apoptosis and disordered polarization of peripheral blood T cells in ankylosing spondylitis might involve in the immunopathogenesis of ankylosing spondylitis .