| Experimental Study of Effect of Corticosterone on Primary Cultured Hippocampal Neurons and Their Ca^2＋／CaMK Ⅱ Expression |
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| 引用本文： | 孙臣友 刘能保 李宏莲 张敏海 刘少纯 刘向前 李晓恒 洪小平. Experimental Study of Effect of Corticosterone on Primary Cultured Hippocampal Neurons and Their Ca^2＋／CaMK Ⅱ Expression[J]. 华中科技大学学报(医学英德文版), 2004, 24(6): 543-546. DOI: 10.1007/BF02911349 |
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| 作者姓名： | 孙臣友 刘能保 李宏莲 张敏海 刘少纯 刘向前 李晓恒 洪小平 |
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| 作者单位： | SectionofHistolongyandEmbryolongy,DepartmentofAnatomy.TongjiMedicalCollege.HuazhongUniversityofScienceandTechnology,Wuhan430030,China |
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| 基金项目： | agrantfromtheNationalNaturalSciencesFoundationofChina(No.30270446) |
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| 摘 要： | Summary: To explore the effect of different concentrations of corticosterone (CORT) on primary cultured hippocampal neurons and their Ca^2 /CaMK Ⅱ expression and possible mechanism, the changes of hippocampal neurons were observed in terms of morphology, activity of cells, cell death.concentrations of cytosolic free calcium, and the expression of CaMK Ⅱ by using MTT assay, flow cytometry, fluorescent labeling of Fura-2/AM and Western hlotting after 10^-7. 10^-8 and 10^-5 mol/L of CORT was added to culture medium. The evident effect of 10^-6 and 10^-5 mol/L of CORT on the morphology of hippocampal neuron was found. Compared with control neurons, the activity of the cells was markedly decreased and [Ca^2 ], increased in the neurons treated with 10^-6 and 10^-7mol/L of CORT. but no change was observed in the neuron treated with 10^-7 mol/L of CORT.The death was either by way of apoptosisor necrosis in the cells treated with 10^-4 and 10^-5mol/L of CORT respectively. The correlation analysis showed that a reverse correlation existed between [Ca^2 ];and the expression of CaMKⅡ. Either apoptosis or necrosis occurs in the hippocampal neurons treated with CORT. The increased hippocampal [Ca^2 ], is both the result of CORT impairing the hippocampal neurons and the cause of the apoptosis of hippocampal neurons and the decreased CaMKⅡ expression.
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| 关 键 词： | 试验研究 肾上腺酮 海马趾神经元 Ca^2＋ CaMKⅡ 基因表达 皮质酮 CORT |
Experimental study of effect of corticosterone on primary cultured hippocampal neurons and their Ca2+/CaMK II expression |
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| Sun?Chenyou,Liu?NengbaoEmail author,Li?Honglian,Zhang?Minhai,Liu?Shaochun,Liu?Xiangqian,Li?Xiaoheng,Hong?Xiaoping. Experimental study of effect of corticosterone on primary cultured hippocampal neurons and their Ca2+/CaMK II expression[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2004, 24(6): 543-546. DOI: 10.1007/BF02911349 |
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| Authors: | Sun?Chenyou,Liu?Nengbao author-information" > author-information__contact u-icon-before" > mailto:Liunb@sina.com.cn" title=" Liunb@sina.com.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Li?Honglian,Zhang?Minhai,Liu?Shaochun,Liu?Xiangqian,Li?Xiaoheng,Hong?Xiaoping |
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| Affiliation: | Section of Histology and Embryology, Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Liunb367@sina.com.cn |
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| Abstract: | To explore the effect of different concentrations of corticosterone (CORT) on primary cultured hippocampal neurons and their Ca2+/CaMK II expression and possible mechanism, the changes of hippocampal neurons were observed in terms of morphology, activity of cells, cell death, concentrations of cytosolic free calcium, and the expression of CaMK II by using MTT assay, flow cytometry, fluorescent labeling of Fura-2/AM and Western blotting after 10(-7), 10(-6) and 10(-5) mol/L of CORT was added to culture medium, The evident effect of 10(-6) and 10(-5) mol/L of CORT on the morphology of hippocampal neuron was found. Compared with control neurons, the activity of the cells was markedly decreased and [Ca2+]i increased in the neurons treated with 10(-6) and 10(-5) mol/L of CORT, but no change was observed in the neuron treated with 10(-7) mol/L of CORT. The death was either by way of apoptosis or necrosis in the cells treated with 10(-6) and 10(-5) mol/L of CORT respectively. The correlation analysis showed that a reverse correlation existed between [Ca2+]i and the expression of CaMK II. Either apoptosis or necrosis occurs in the hippocampal neurons treated with CORT. The increased hippocampal [Ca2+]i is both the result of CORT impairing the hippocampal neurons and the cause of the apoptosis of hippocampal neurons and the decreased CaMK II expression. |
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| Keywords: | corticosterone hippocampal neurons Ca~(2 ) CaMKII |
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