组蛋白去乙酰化酶抑制剂蛋白去乙酰化酶抑制剂对原发性肝癌细胞增殖的影响

目的 观察组蛋白去乙酰化酶(HDAC)抑制剂蛋白去乙酰化酶抑制剂(TSA)对原发性肝癌细胞增殖的影响.方法 噻唑蓝(MTT)法检测不同浓度(30、150、1000μg/L)TSA对原发性肝癌细胞生长的影响.结果 TSA浓度为30μg/L时,实验组与对照组细胞的吸光度值差异无统计学意义(P>0.05),说明TSA(30μg/L)对细胞的增殖基本无影响;当TSA浓度达到1000μg/L时,实验组细胞大量崩解、坯死,对细胞产生了严重的毒性作用;当TSA浓度为150μg/L时,实验组细胞无崩解、坏死现象,其36h和48h细胞的吸光度值低于对照组,差异有统计学意义(P<0.05).结论 HDAC抑制剂TSA对体外培养的原发性肝癌细胞的增殖生长具有抑制作用,并且这种抑制作用呈现一定剂量依赖性和时间依赖性.

Effect of histone deacetylase inhibitors on proliferation of hepatocelluar carcinoma cells

Objective To study the effect of histone deacetylase inhibitors (TSA) on proliferation of hepatocelluar carcinoma (HCC) cells.Methods MTT assay was used to evaluate the inhibitory effect of TSA (30,150,1000μg/L) on HCC cell line BEL-7402.Results There were no obvious changes in proliferation after HCC cells were treated with 30μg/L TSA.1000μg/L TSA showed severe cytotoxicity.150μg/L TSA inhibited proliferation of HCC cells.Conclusion After treatment with TSA (150μg/L),proliferation of HCC cell line BEL-7402 cells can be inhibited.