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Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
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| Authors: | Sheng Huang Kangli Xu Shanshan Xu Shijun Yu Yan Miao Juan Wang |
| Institution: | 1.Department of Laboratory Medicine, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China; 2.Department of Hematology and Oncology, Shanghai Children’s Medical Center, Shanghai JiaoTong University School of Medicine, Shanghai, China; 3.Department of Clinical Laboratory, Shanghai Tenth People’s Hospital of Tongji University, Shanghai, China; 4.BD Biosciences, 2350 Qume Drive, San Jose, CA, USA |
| Abstract: | ObjectiveTo assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences.MethodsCord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software.ResultsA total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively.ConclusionsThe hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples. |
| Keywords: | Flow cytometry CD34 stem cell cord blood stem cell enumeration kit BD Biosciences |