| 反相高效液相色谱法测定人血浆和尿中丹皮酚浓度 |
| |
| 引用本文： | 吴秀君,李国信,肇丽梅.反相高效液相色谱法测定人血浆和尿中丹皮酚浓度[J].中国药学杂志,2007,42(11):863-865. |
| |
| 作者姓名： | 吴秀君 李国信 肇丽梅 |
| |
| 作者单位： | 1. 中国医科大学附属第二医院临床药理研究室,沈阳,110004
2. 辽宁中医药火学附属第二医院,沈阳,110042 |
| |
| 摘 要： | 目的建立测定血浆、尿样本中丹皮酚含量的反相高效液相色谱法。方法血浆样品经沉淀蛋白,尿样经稀释后,以乙腈-0.1%磷酸pH3.5(55∶45)为流动相,经Diamonsil C18柱分离,进行色谱分析。结果本方法丹皮酚的血浆浓度在20～1000μg·L-1,尿中浓度在20～800μg·L-1内线性关系良好,方法回收率在81.0%～85.0%之间,日内、日间RSD均小于11.0%。结论本方法简便、快速、灵敏度高,可用于丹皮酚的药动学研究。
|
| 关 键 词： | 反相高效液相色谱 丹皮酚 人血浆 人尿样 |
| 文章编号： | 1001-2494（2007）11-0863-03 |
| 收稿时间： | 2006-09-20; |
| 修稿时间： | 2006-09-20 |
Determination of Paeonol in Plasma and Urine by RP-HPLC |
| |
| WU Xiu-jun,LI Guo-xin,ZHAO Li-mei.Determination of Paeonol in Plasma and Urine by RP-HPLC[J].Chinese Pharmaceutical Journal,2007,42(11):863-865. |
| |
| Authors: | WU Xiu-jun LI Guo-xin ZHAO Li-mei |
| |
| Institution: | 1. Clinical Pharmacology Laboratory, Second Hospital of China Medical University, Shenyang 110004, China ;2. Second Hospital of Traditional Chinese Medicines University, Shenyang 110042, China |
| |
| Abstract: | OBJECTIVE To establish a method for the determination of paeonol concentration in plasma and in urine. METHODS The analysis was conducted on a Diamonsil-C18 (4.6 mm×150 mm,5 μm) column with mobile phase consisting of acetonitrile-0.1% phosphate acid (55∶45,adjusted to pH 3.5 with triethylamine). RESULTS Good linearity was obtained in the concentration range of 20～1 000 μg·L-1of paeonol in plasma and 20～800 μg·L-1 in urine. The average recovery was 81.0%～85.0% at three different concentrations. The RSDs of intra-and inter-day were less than 11%.CONCLUSION This method is rapid,sensitive,precise,and can be used in the determination of paeonol in plasma and in urine for the clinical pharmacokinetic study. |
| |
| Keywords: | RP-HPLC paeonol human plasma human urine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
| 点击此处可从《中国药学杂志》下载免费的PDF全文 |
|
