中药金果榄两种基源植物的化学成分

目的：研究中药金果榄（Radix Tinosporae）两种基源植物：青牛胆（Tinospont sagittata）和金果榄（Tinospora capillipes）的化学成分，并比较它们物质基础的差别，为中药多基源植物的应用提供证据。方法：青牛胆和金果榄的根分别用95％的乙醇回流提取，在真空下浓缩提供乙醇提取物，乙醇提取物分别用氯仿和正丁醇萃取，氯仿部位和正丁醇部位分别用硅胶、Sephadex LH-20、重结晶技术及反相HPLC半制备液相分离纯化，所得化合物通过它们的波谱数据并与文献值比较进行鉴定，结果：从青牛胆从分离鉴定了12个化合物（1-12）。另外从金果榄中分离鉴定了11个与青牛胆中化合物相同的结构（1-11）。结论：中药金果榄两种基源植物具有类似的化学物质基础。其中化合物10及7-9为首次从金果榄中分离得到。

Chemical Constituents of Two Original Plants Used as Radix Tinosporae

AIM:To study the chemical constituents of two original plants used as TCM Radix Tinosperae,and compare the material foundation of two originaI plants of Radix Tinosporae.METHODS:The roots of Tinospora sagit tata and T. capillipes were refluxed with 95% EtOH,respectively,and the extracts were concentrated to afford ethanol extracts,which were partitioned with CHCl3 and n-Butanol.The fractions obtained were submitted to colnmn ehromatog raphy over silica gel,Sephadex LH-20,and purified by RP-HPLC on a C18 -μBondapak column(7.8 mm×30 cm,flow rate 2.0 mL·min-1 with 30%MeOH and recrystallization (MeOH).The compounds were identified on the basis of their spectroscopic data.RESULTS:Compounds 1-12 were isolated from Tinospora sagittata.and the identical compounds 1-11 from Tinospora capillipes.CONCLUSIONS:Two original plants of TCM Radix Tinosporae have the same effective material foundation based on the similarity of chemical constituents of these two plants.Compounds 1-3 and 7-9 were isolated from T eapillipes for the first time.