COX-2、VEGF-C、Ki67及高危型HPV在宫颈病变中的表达

目的探讨COX-2、VEGF-C、Ki67在宫颈癌(CC)及癌前病变(CIN)组织中的表达及其与高危型HPV感染的相关性,进一步探讨其在宫颈癌发生发展中的意义。方法采用免疫组织化学SABC法检测100例宫颈石蜡切片中COX-2、VEGF-C、Ki67的表达,并于术前采用HC-Ⅱ法检测患者高危型HPV感染情况。结果 (1)COX-2、VEGF-C、Ki67在CC及CIN中的表达水平均高于慢性宫颈炎组织,差异有统计学意义(P0.05);三者在宫颈癌组织中的表达水平与高危型HPV正相关(P0.05);(2)COX-2、VEGF-C分别在CIN1和CC中表达率最高,与其他组差异有统计学意义(P0.05);(3)高危型HPV在CC及CIN中的阳性率均高于慢性宫颈炎组织,差异有统计学意义(P0.05)。结论高危型HPV可能通过诱导宫颈上皮高表达COX-2、VEGF-C而促使宫颈癌发生发展。

Relationship between Expression of COX-2,VEGF-C,Ki67 and HR-HPV in Human Cervical Cancer and Pre-malignant Lesion

Objective To investigate the expression of COX-2,VEGF-C,Ki67 in cervical cancer and pre-malignant lesion,and explore the relationship between the expression and HR-HPV infection. Methods One hundred cases of cervical tissue were collected and the expression levels of COX-2, VEGF-C and Ki67 were detected by immunohistochemical SABC assay and the HR-HPV infection was detected by HC-11 before operations. Results COX-2,VEGF-C and Kit7 were all overexpressed in cervical cancer,compared with those in Chronic inflammation cervical tissue（P〈0.05）. The HR-HPV infection was assessed at all stages of cervical pre-malignant lesion samples,and there was a positive correlation between the expression of COX-2,VEGF-C and Ki67 in the cervical tissue and the HR-HPV infection. In cervical cancer samples, the expression levels of VEGF-C and Ki67 seemed to be more closely associated with HR-HPV infection than with COX-2 （r=-0.7 vs r=-0.3）. The COX-2 overexpression was observed in CIN1 （P〈0.05）and the VEGF-C and Ki67 overexpression in cervical cancer samples（P〈0.05）. Conclusion In cervical cancer, the expression of COX-2,VEGF-C and Ki67 were positively correlated with HR-HPV infection. COX-2 and VEGF-C may play a vital role in the HR-HPV may cause the occurrence and development of cervical cancer by inducing high expression of COX-2 and VEGF-C in the cervical intraepithelial tissue.