| dentification of differentially expressed genes from spinal cord after firearm injury to rabbit sciatic nerves |
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| 作者姓名： | 王建民 李兵仓 陈蕾 王贵波 张良朝 |
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| 作者单位： | ResearchInstituteofSurgery,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China. |
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| 摘 要： | Objective: Peripheral nerve regeneration depends on gene regulation by central neurons. To search for more effective treatment methods to improve the regeneration of wounded peripheral nerves, gene expression profile of spinal cord after firearm injury to rabbit sciatic nerves are studied with DNA micro-array technique. Methods: A total of 54 rabbits were randomly divided into 4 groups: Groups dl, d3, d7 and normal control group. Lumbar spinal cords were sampled. RNAand mRNA were extracted, labeled by Cy3 and Cy5, and analyzed by mouse_8192S gene chips. Results: A total of 1367, 923, and 61 genes with differential expression were found on day 1, day 3, and day 7 after trauma respectively. Five expressed sequence tag (EST) sequences demonstrated differential expression during 7 days after trauma. Conclusions: There is complex gene profile with differential expression after firearm nerve injury, among which AW701496, U84291, W13926, X04017 andAW822394 EST sequences may be important regulation factors that involved in regeneration of peripheral nerve injury.
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| 关 键 词： | 鉴定法 差异表达 基因表达 脊椎麻醉 火器伤害 兔 髋部神经 外伤 |
Identification of differentially expressed genes from spinal cord after firearm injury to rabbit sciatic nerves. |
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| Jian-Min Wang,Bing-Cang Li,Lei Chen,Gui-Bo Wang,Liang-Chao Zhang.dentification of differentially expressed genes from spinal cord after firearm injury to rabbit sciatic nerves[J].Chinese Journal of Traumatology(English Edition),2004,7(5):294-300. |
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| Authors: | Jian-Min Wang Bing-Cang Li Lei Chen Gui-Bo Wang Liang-Chao Zhang |
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| Institution: | Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. jmwang@public.cta.cq.cn |
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| Abstract: | OBJECTIVE: Peripheral nerve regeneration depends on gene regulation by central neurons. To search for more effective treatment methods to improve the regeneration of wounded peripheral nerves, gene expression profile of spinal cord after firearm injury to rabbit sciatic nerves are studied with DNA micro-array technique. METHODS: A total of 54 rabbits were randomly divided into 4 groups: Groups d1, d3, d7 and normal control group. Lumbar spinal cords were sampled. RNA and mRNA were extracted, labeled by Cy3 and Cy5, and analyzed by mouse_8192S gene chips. RESULTS: A total of 1367, 923, and 61 genes with differential expression were found on day 1, day 3, and day 7 after trauma respectively. Five expressed sequence tag (EST) sequences demonstrated differential expression during 7 days after trauma. CONCLUSIONS: There is complex gene profile with differential expression after firearm nerve injury, among which AW701496, U84291, W13926, X04017 and AW822394 EST sequences may be important regulation factors that involved in regeneration of peripheral nerve injury. |
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