| 高效液相色谱法测定牛樟芝中腺苷的含量 |
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| 引用本文： | 程利娟,;郭琪,;雷虹,;杨莹,;申睿,;慕磊,;王彦宗. 高效液相色谱法测定牛樟芝中腺苷的含量[J]. 华北国防医药, 2014, 0(5): 76-78 |
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| 作者姓名： | 程利娟, 郭琪, 雷虹, 杨莹, 申睿, 慕磊, 王彦宗 |
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| 作者单位： | [1]河北北方学院药理教研室,河北张家口075000; [2]北京军区联勤部药品仪器检验所,北京100071 |
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| 基金项目： | 军队中医药科研专项课题（IOZYZ204） |
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| 摘 要： | 目的：采用高效液相色谱法测定牛樟芝中腺苷的含量。方法色谱柱：Agilent Zorbax SB-C18柱（4.6 mm ×250 mm,5μm）,流动相：乙腈-0.01 mol/L磷酸二氢钾溶液（6∶94）,流速：1.0 ml/min,检测波长：260 nm,柱温：30℃,进样量：10μl。结果腺苷在2.696~67.40 g/ml范围内与其峰面积呈现良好的线性关系（r=0.9999）,平均回收率为99.89%,相对标准偏差为1.71%（n=6）。结论该方法简便准确,灵敏度高,重复性好,为控制牛樟芝的质量提供了科学依据。
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| 关 键 词： | 牛樟芝 腺苷 色谱法,高效液相 |
High Performance Liquid Chromatography in Determination of Adenosine Content in Antrodia Camphorata |
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| Affiliation: | CHENG Li-juan, GUO Qi, LEI Hong, YANG Ying, SHEN Rui, MU Lei, WANG Yan-zong (1. Department of Pharmacology, Hebei North University, Zhangjiakou, Hebei 075000, China; 2. Institute for Drug and Instrument Con- trol, Combined Logistics Department of Beijing Military Area Command, Beijing 100071, China) |
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| Abstract: | Objective To detect the Adenosine content in Antrodia Camphorata using high performance liquid chromatography （HPLC）. Methods The chromatographic column was Agilent Zorbax SB-C18 column （4. 6 mm × 250 mm, 5 μm）; the mobile phase was acetonitrile-0. 01 mol＆#183;L-1 KH2PO4 solution （6∶ 94）; the flow rate was 1. 0 ml/min-1; the detection wavelength was 260 nm;the column temperature was set at 30℃;the injection volume was 10 μl. Results A good linear relationship between the peak area and Adenosine concentration was observed within the range of 2. 696-67. 40 mg/ml （r=0. 9999）. The average recovery was 99. 89% with 1. 71% of relative standard devia-tion （n=6）. Conclusion This method is accurate and has good sensitivity and reproducibility, and can provide scien-tific basis for controlling the quality of Antrodia camphorata. |
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| Keywords: | Antrodia camphorata Adenosine Chromatography,high performance liquid |
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